Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 30% v/v, 5fold activation | Thermus thermophilus | |
propan-1-ol | activates | Thermus thermophilus | |
propan-2-ol | activates | Thermus thermophilus | |
tetrabutylammonium chloride | 50% activation | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
General Stability | Organism |
---|---|
8 M urea, 40% inactivation | Thermus thermophilus |
repeated freezing and thawing brings about 10-20% inactivation | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | - |
Thermus thermophilus | |
Cu2+ | - |
Thermus thermophilus | |
HgCl2 | - |
Thermus thermophilus | |
iodoacetamide | 0.5 mM, 9% inhibition | Thermus thermophilus | |
iodoacetate | 0.2 mM, 67% inhibition | Thermus thermophilus | |
Na2S4O6 | 0.1 mM, complete inhibition | Thermus thermophilus | |
NiCl2 | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | - |
Thermus thermophilus | |
0.3 | - |
D-glyceraldehyde 3-phosphate | - |
Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 0.045 M, 10fold activation | Thermus thermophilus | |
CsCl | activates | Thermus thermophilus | |
CsCl | maximal activation: 20fold | Thermus thermophilus | |
K+ | maximal activation: 22fold | Thermus thermophilus | |
LiCl | maximal activation: 10fold | Thermus thermophilus | |
Na2SO4 | up to 5fold activation | Thermus thermophilus | |
NH4Cl | 0.1 M, 25fold activation | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33700 | - |
4 * 33700, SDS-PAGE | Thermus thermophilus |
128000 | - |
equilibrium sedimentation | Thermus thermophilus |
135000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB 8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Thermus thermophilus |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 years | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus thermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus thermophilus HB 8 | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33700, SDS-PAGE | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, stable | Thermus thermophilus |
90 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 20% loss of acticity | Thermus thermophilus |
95 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 85% loss of acticity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | pH 7.0: about 55% of maximal activity, pH 8.8: about 95% of maximal activity | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |