Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | maximal activation at 5 mM, dependent on sulfhydryl compound | Haloarcula vallismortis | |
GSH | dependent on sulfhydryl compound | Haloarcula vallismortis |
General Stability | Organism |
---|---|
very stable in 4 M KCl. Inactivated within 12 h in 0.5 M KCl, 50 mM Tris-HCl, pH 8.0, at 25°C | Haloarcula vallismortis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | - |
Haloarcula vallismortis | |
Cu2+ | 1 mM CuCl2, complete inhibition | Haloarcula vallismortis | |
HgCl2 | 1 mM, complete inhibition | Haloarcula vallismortis | |
iodoacetamide | 5 mM, complete inhibition | Haloarcula vallismortis | |
PCMB | 5 mM, complete inhibition | Haloarcula vallismortis | |
pentalenolactone | insensitive to | Haloarcula vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
D-glyceraldehyde 3-phosphate | - |
Haloarcula vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | KCl, activity is enhanced by increasing concentrations of the salt | Haloarcula vallismortis | |
LiCl | activity is partly enhanced | Haloarcula vallismortis | |
NaCl | activity is enhanced by increasing concentrations of the salt | Haloarcula vallismortis | |
NH4Cl | activity is enhanced by increasing concentrations of the salt | Haloarcula vallismortis | |
RbCl | activity is enhanced by increasing concentrations of the salt. Optimum activity in 3 M RbCl | Haloarcula vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Haloarcula vallismortis |
190000 | - |
gel filtration | Haloarcula vallismortis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Haloarcula vallismortis | key enzyme of glycolysis | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula vallismortis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula vallismortis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.5 | - |
- |
Haloarcula vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Haloarcula vallismortis | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | key enzyme of glycolysis | Haloarcula vallismortis | 3-phospho-D-glyceroyl phosphate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Haloarcula vallismortis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature of 50% inactivation increases by 15°C, when KCl concentration is increased from 1 M to 4 M or when 1 M KCl is replaced by 1 M (NH4)2SO4 | Haloarcula vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Haloarcula vallismortis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Haloarcula vallismortis |