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Literature summary for 1.2.1.12 extracted from

  • Zammit, A.; Copeland, L.
    Purification and immunological properties of NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from the plant cytosol of nitrogen fixing soybean nodules (1991), J. Plant Physiol., 138, 44-50.
No PubMed abstract available
Search for more literature for 1.2.1.12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 4 * 37000, SDS-PAGE Glycine max
147000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
 CB1809
-
Bradyrhizobium japonicum CB1809
-
 CB1809
-
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
bacteroid
-
 Bradyrhizobium japonicum
-
nodule nitrogen fixing Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145
-
-
 Glycine max

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.4, stable for at least 2 weeks Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bradyrhizobium japonicum 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Glycine max 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bradyrhizobium japonicum CB1809 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE Glycine max

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bradyrhizobium japonicum
NAD+
-
 Glycine max