Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Agaricic acid | 0.14 mM, 50% inhibition in absence of NAD+. 2 mM, 35% loss of activity in presence of 0.017 mM NAD+ | Oryctolagus cuniculus | |
Agaricic acid | glyoxysomal enzyme: 0.2 mM, 60% loss of activity. Cytosolic enzyme: 2 mM, less than 20% loss of activity | Trypanosoma brucei | |
gossypol | - |
Trypanosoma brucei | |
pentalenolactone | reversible | Geobacillus stearothermophilus | |
pentalenolactone | irreversible | Oryctolagus cuniculus | |
pentalenolactone | most potent inhibitor; reversible | Trypanosoma brucei | |
suramin | cyosolic enzyme: competitive with NAD+. Effect on Km-value and maximal veocity of glyoxysomal enzyme | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADH | cytosolic enzyme | Trypanosoma brucei | |
0.01 | - |
NADH | - |
Homo sapiens | |
0.012 | - |
NADH | - |
Oryctolagus cuniculus | |
0.012 | - |
NADH | - |
Geobacillus stearothermophilus | |
0.02 | - |
NADH | glyoxysomal enzyme | Trypanosoma brucei | |
0.034 | - |
3-phospho-D-glyceroyl phosphate | - |
Geobacillus stearothermophilus | |
0.039 | - |
NAD+ | - |
Geobacillus stearothermophilus | |
0.04 | - |
NAD+ | cytosolic enzyme | Trypanosoma brucei | |
0.05 | - |
NAD+ | - |
Homo sapiens | |
0.05 | - |
NAD+ | - |
Geobacillus stearothermophilus | |
0.06 | - |
NAD+ | - |
Oryctolagus cuniculus | |
0.07 | - |
D-glyceraldehyde 3-phosphate | - |
Homo sapiens | |
0.082 | - |
D-glyceraldehyde 3-phosphate | - |
Oryctolagus cuniculus | |
0.09 | - |
D-glyceraldehyde 3-phosphate | - |
Oryctolagus cuniculus | |
0.09 | - |
D-glyceraldehyde 3-phosphate | - |
Geobacillus stearothermophilus | |
0.1 | - |
3-phospho-D-glyceroyl phosphate | glyoxysomal enzyme | Trypanosoma brucei | |
0.13 | - |
3-phospho-D-glyceroyl phosphate | - |
Oryctolagus cuniculus | |
0.14 | - |
3-phospho-D-glyceroyl phosphate | - |
Homo sapiens | |
0.14 | - |
3-phospho-D-glyceroyl phosphate | cytosolic enzyme | Trypanosoma brucei | |
0.15 | - |
D-glyceraldehyde 3-phosphate | glyoxysomal enzyme | Trypanosoma brucei | |
0.17 | - |
D-glyceraldehyde 3-phosphate | cytosolic enzyme | Trypanosoma brucei | |
0.45 | - |
NAD+ | glyoxysomal enzyme | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma brucei | 5829 | - |
glyoxysome | - |
Trypanosoma brucei | 9514 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cytosolic isoenzyme | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
muscle | - |
Homo sapiens | - |
muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
additional information | - |
- |
Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH | - |
Homo sapiens | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
3-phospho-D-glyceroyl phosphate + NADH | - |
Oryctolagus cuniculus | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
3-phospho-D-glyceroyl phosphate + NADH | - |
Geobacillus stearothermophilus | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
3-phospho-D-glyceroyl phosphate + NADH | - |
Trypanosoma brucei | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Homo sapiens | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Oryctolagus cuniculus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Geobacillus stearothermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Trypanosoma brucei | 3-phospho-D-glyceroyl phosphate + NADH | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | cytosolic enzyme and enzyme from glyoxysomes | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Oryctolagus cuniculus | |
NAD+ | - |
Geobacillus stearothermophilus | |
NAD+ | - |
Trypanosoma brucei | |
NADH | cofactor | Homo sapiens | |
NADH | cofactor | Oryctolagus cuniculus | |
NADH | cofactor | Geobacillus stearothermophilus | |
NADH | cofactor | Trypanosoma brucei |