Any feedback?
Literature summary for 1.2.1.11 extracted from
- A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii (2005), J. Mol. Biol., 353, 1055-1068.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
| into the pET41-41a vector for transformation into Rosetta-competent Escherichia coli cells | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 2.3 A resolution | Methanocaldococcus jannaschii |
| the structure of aspartate-beta-semialdehyde dehydrogenase has been determined to 2.3 A resolution using multiwavelength anomalous diffraction phasing of a seleno-methionine-substituted derivative | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-phosphoribose AMP | - |
Methanocaldococcus jannaschii |  |
| ADP | - |
Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADP+ | - |
Methanocaldococcus jannaschii | |
| 1.7 | - |
NAD+ | - |
Methanocaldococcus jannaschii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate 4-semialdehyde + phosphate + NADP+ | Methanocaldococcus jannaschii | - |
L-4-aspartyl phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange chromatography and gel-filtration | Methanocaldococcus jannaschii |
| using anion-exchange chromatography, Source 15Q, followed by gel filtration | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate 4-semialdehyde + phosphate + NAD+ | - |
Methanocaldococcus jannaschii | L-4-aspartyl phosphate + NADH + H+ | - |
? | |
| L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Methanocaldococcus jannaschii | L-4-aspartyl phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | crystallization | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASADH | - |
Methanocaldococcus jannaschii |
| aspartate-beta-semialdehyde dehydrogenase | - |
Methanocaldococcus jannaschii |
| aspartate-semialdehyde dehydrogenase | - |
Methanocaldococcus jannaschii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Methanocaldococcus jannaschii | |
| NADPH | - |
Methanocaldococcus jannaschii | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
2'-phosphoribose AMP | - |
Methanocaldococcus jannaschii | |
| 0.4 | - |
ADP | - |
Methanocaldococcus jannaschii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
