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Literature summary for 1.2.1.11 extracted from

  • Paris, S.; Wessel, P.M.; Dumas, R.
    Overproduction, purification, and characterization of recombinant aspartate semialdehyde dehydrogenase from Arabidopsis thaliana (2002), Protein Expr. Purif., 24, 99-104.
    View publication on PubMed
Search for more literature for 1.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
cloning and recombinant protein overproduction with Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0929
-
 NADPH at 0.15 mM aspartyl phosphate Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH Arabidopsis thaliana
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FVC4
-
-

Purification (Commentary)

Purification (Comment) Organism
using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
-
 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-80°C, no noticeable loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
L-4-aspartyl phosphate + NADPH + H+
-
 Arabidopsis thaliana L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Arabidopsis thaliana L-4-aspartyl phosphate + NADPH
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.6
-
 L-4-aspartyl phosphate
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Arabidopsis thaliana