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Literature summary for 1.2.1.11 extracted from

  • Biellmann, J.F.; Eid, P.; Hirth, C.; Jörnvall, H.
    Aspartate-beta-semialdehyde dehydrogenase from Escherichia coli. Purification and general properties (1980), Eur. J. Biochem., 104, 53-58.
    View publication on PubMed
Search for more literature for 1.2.1.11

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, SDS-PAGE Escherichia coli
77500
-
 ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-4-aspartyl phosphate + NADPH Escherichia coli enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 mutant derepressed for aspartate-beta-semialdehyde dehydrogenase biosynthesis
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, hydroxylapatite, ultrogel ACA-44 and Sepharose C3 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100 110
-
 Escherichia coli
105
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, 50% glycerol, 2 months Escherichia coli
4°C, 10 mM potassium phosphate, pH 7.2, 0.1 mM EDTA, 1 mM dithioerythritol, several days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-aspartyl phosphate + NADPH enzyme in pathway from L-aspartic acid to L-lysine, L-methionine, L-threonine and L-isoleucine Escherichia coli L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Escherichia coli L-4-aspartyl phosphate + NADPH
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADPH L-aspartate 4-semialdehyde dehydrogenase transfers the pro-S hydrogen from NADPH Escherichia coli