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Literature summary for 1.2.1.107 extracted from
- [55] Glyceraldehyde-3-phosphate dehydrogenase (glycolytic form) from spinach leaves (1982), Methods Enzymol., 89, 316-319 .
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Spinacia oleracea | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Spinacia oleracea | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, acetone precipitation, NAD-Sepharose column chromatography, and Sephadex G-100 gel filtration, and DEAE-Sephadex gel filtration | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 80.4 | - |
purified enzyme, at pH 8.5 and 25°C | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, purified enzyme, 6 months, about 70% loss of activity | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Spinacia oleracea | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 37000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | 9.1 | - |
Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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