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Literature summary for 1.2.1.107 extracted from
- Expression, purification, enzymatic characterization and crystallization of glyceraldehyde-3-phosphate dehydrogenase from Naegleria gruberi, the first one from phylum Percolozoa (2016), Protein Expr. Purif., 127, 125-130 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Naegleria gruberi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using either 12.9% (w/w) PEG 2000, 50 mM acetate buffer (pH 5.2) and 6.4% (w/v) PEG 200 or2.5% (w/v) PEG 1000, 50 mM acetate buffer (pH 4.8) and 25.7% (w/v) PEG 2000 MME | Naegleria gruberi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Naegleria gruberi | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Naegleria gruberi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography and Superdex 75 gel filtration | Naegleria gruberi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Naegleria gruberi | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAPDH | - |
Naegleria gruberi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Naegleria gruberi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | high activity between 20 and 30°C | Naegleria gruberi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Naegleria gruberi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | high activity between pH 7.5 and 8.5 | Naegleria gruberi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Naegleria gruberi | |
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