Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | enhances the rate of succinylation of the enzyme complex | Komagataeibacter xylinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | prevents desuccinylation of the 2-oxoglutarate dehydrogenase complex | Komagataeibacter xylinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
2600000 Da is the MW of the 2-oxoglutarate dehydrogenase complex, calculation from sedimentation and diffusion data | Komagataeibacter xylinus |
100000 | - |
4 * 100000, active 2-oxoglutarate dehydrogenase component, SDS-PAGE | Komagataeibacter xylinus |
360000 | - |
active 2-oxoglutarate dehydrogenase component, gel filtration | Komagataeibacter xylinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataeibacter xylinus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | 2-oxoglutarate dehydrogenase complex consists of 3 enzymes: E1 (alpha-ketoglutarate dehydrogenase, EC 1.2.4.2), E2 (dihydrolipoyl transsuccinylase, EC 2.3.1.61), E3 (dihydrolipoyl dehydrogenase, EC 1.8.1.4) | Komagataeibacter xylinus | |
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH | Komagataeibacter xylinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + lipoamide | - |
Komagataeibacter xylinus | S-succinyldihydrolipoamide + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the complex constists of 12 chains of E1, EC 1.2.4.2, 12 chains of E2, EC 2.3.1.61 and 12 chains of E3, EC 1.8.1.4 | Komagataeibacter xylinus |
tetramer | 4 * 100000, active 2-oxoglutarate dehydrogenase component, SDS-PAGE | Komagataeibacter xylinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the complex contains 12 lipoyl groups | Komagataeibacter xylinus | |
additional information | the complex contains 12.1 mol FAD per mol of enzyme complex | Komagataeibacter xylinus |