Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidinium chloride | treatment of the E2-X subcomplex with 4 M guanidinium chloride causes a complete loss of enzymatic activity and the dissociation of the subcomplex into monomeric 1.5-3 S species. Removal of the chaotrope by dialysis for 18 h results in complete restoration of E2 enzymatic activity and reassembly of a 32 S subcomplex. The reassembled E2-X subcomplex demonstrates the presence of an 8 S assembly intermediate. The 8 S species associates non-cooperatively to yield additional assembly intermediates exhibiting sedimentation coefficients of 10-32 S | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
treatment of the E2-X subcomplex with 4 M guanidinium chloride causes a complete loss of enzymatic activity and the dissociation of the subcomplex into monomeric 1.5-3 S species. Removal of the chaotrope by dialysis for 18 h results in complete restoration of E2 enzymatic activity and reassembly of a 32 S subcomplex | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |