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Literature summary for 1.2.1.104 extracted from

  • Millar, A.; Knorpp, C.; Leaver, C.; Hill, S.
    Plant mitochondrial pyruvate dehydrogenase complex Purification and identification of catalytic components in potato (1998), Biochem. J., 334, 571-576 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.104

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitive Solanum tuberosum
NADH competitive Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 CoA pH 7.5, 25°C Solanum tuberosum
0.062
-
 pyruvate pH 7.5, 25°C Solanum tuberosum
0.27
-
 NAD+ pH 7.5, 25°C Solanum tuberosum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Solanum tuberosum 5739
-

Organism

Organism UniProt Comment Textmining
Solanum tuberosum P52903 and P80503 P52903 i.e. E1 component subunit alpha, P80503 i.e. dihydrolipoyl dehydrogenase, cf. EC 1.8.1.4
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
 pH 7.5, 25°C Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + CoA + NAD+
-
 Solanum tuberosum acetyl-CoA + CO2 + NADH
-
 ?

Subunits

Subunits Comment Organism
multimer x * 78000, i.e. dihydrolipoamide transacetylase (E2) subunit, x * 60000, x * 58000, i.e. dihydrolipoamide dehydrogenase (E3) subunit, x * 55000, x * 43000 and x * 41000, i.e. alpha-subunits of pyruvate dehydrogenase, and x * 37000, i.e. beta-subunit of pyruvate dehydrogenase (E1), respectively, SDS-PAGE Solanum tuberosum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.024
-
 NADH pH 7.5, 25°C Solanum tuberosum
0.079
-
 acetyl-CoA pH 7.5, 25°C Solanum tuberosum