Literature summary for 1.2.1.104 extracted from

Buchholz, J.; Schwentner, A.; Brunnenkan, B.; Gabris, C.; Grimm, S.; Gerstmeir, R.; Takors, R.; Eikmanns, B.J.; Blombach, B.
Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate (2013), Appl. Environ. Microbiol., 79, 5566-5575 .

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Application

Application	Comment	Organism
synthesis	exchange of the native Corynebacterium glutamicum promoter of the AceE gene, with mutated DapA promoter variants leads to a series of strains with gradually reduced growth rates and pyruvate dehydrogenase complex activities. Upon overexpression of the L-valine biosynthetic genes IlvBNCE, all strains produce L-valine. Additional deletion of the Pqo and Ppc genes, encoding pyruvate:quinone oxidoreductase and phosphoenolpyruvate carboxylase enables production of up to 738mM (i.e., 86.5 g/liter). Inactivation of the transaminase B gene (IlvE) and overexpression of IlvBNCD instead of ilvBNCE transform the L-valine-producing strain into a 2-ketoisovalerate producer, excreting up to 303mM (35 g/liter) 2-ketoisovalerate. The replacement of the AceE promoter by the DapA-A16 promoter improves the production by 100% and 44%, respectively	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	Q8NNF6	Q8NNF6 i.e. E1 component AceE, cf. EC 1.2.4.1	-

Synonyms

Synonyms	Comment	Organism
aceE	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)