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Literature summary for 1.2.1.104 extracted from
- The composition of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Does a unifying model exist for the complexes from gram-negative bacteria? (1984), Eur. J. Biochem., 142, 541-549.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
2 * 94000, SDS-PAGE | Azotobacter vinelandii |
| 190000 | - |
sedimentation equilibrium centrifugation | Azotobacter vinelandii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Azotobacter vinelandii |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of pyruvate dehydrogenase complex | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | 19 | - |
Azotobacter vinelandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| liquid N2, 50 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 0.05 mM phenylmethanesulfonylfluoride, 20-40 mg/ml protein | Azotobacter vinelandii |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 94000, SDS-PAGE | Azotobacter vinelandii |
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