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Literature summary for 1.2.1.104 extracted from
- Purification of the chloroplast pyruvate dehydrogenase complex from spinach and maize mesophyll (1986), Z. Naturforsch. C, 41, 1011-1017.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very sensitive to freezing and thawing | Spinacia oleracea |
| very sensitive to freezing and thawing | Pisum sativum |
| very sensitive to freezing and thawing | Zea mays |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | - |
Pisum sativum | 9507 | - |
| chloroplast | - |
Zea mays | 9507 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + lipoamide | Spinacia oleracea | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | S-acetyldihydrolipoamide + CO2 | - |
? |  |
| pyruvate + lipoamide | Pisum sativum | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | S-acetyldihydrolipoamide + CO2 | - |
? | |
| pyruvate + lipoamide | Zea mays | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | S-acetyldihydrolipoamide + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
| Spinacia oleracea | - |
spinach | - |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Spinacia oleracea |
| pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00085 | - |
enzyme from chloroplast | Spinacia oleracea |
| 0.00265 | - |
enzyme from chloroplast | Zea mays |
| 0.00485 | - |
enzyme from mitochondrion | Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM glycyl-glycine buffer, pH 8.0, 2 mM MgCl2, 2 mM DTT, 1-2 weeks | Spinacia oleracea |
| -20°C, 50 mM glycyl-glycine buffer, pH 8.0, 2 mM MgCl2, 2 mM DTT, 1-2 weeks | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + lipoamide | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | Spinacia oleracea | S-acetyldihydrolipoamide + CO2 | - |
? | |
| pyruvate + lipoamide | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | Pisum sativum | S-acetyldihydrolipoamide + CO2 | - |
? | |
| pyruvate + lipoamide | in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate | Zea mays | S-acetyldihydrolipoamide + CO2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
| additional information | - |
value for pyruvate dehydrogenase complex: 8.2, chloroplast enzyme | Spinacia oleracea |
| additional information | - |
value for pyruvate dehydrogenase complex: 8.2, chloroplast enzyme | Zea mays |
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Release 2023.1 (January 2023)
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