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Literature summary for 1.2.1.10 extracted from

  • Tsuji, K.; Yoon, K.S.; Ogo, S.
    Biochemical characterization of a bifunctional acetaldehyde-alcohol dehydrogenase purified from a facultative anaerobic bacterium Citrobacter sp. S-77 (2016), J. Biosci. Bioeng., 121, 253-258.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.5
-
acetaldehyde pH 7.0, 30°C Citrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Citrobacter sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
4 * 96000, SDS-PAGE Citrobacter sp.
400000
-
gel filtration Citrobacter sp.

Organism

Organism UniProt Comment Textmining
Citrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ADHE cannot be solubilized from membrane with detergents such as 1% Triton X-100 or 1% sulfobetaine 3-12. The enzyme is easily dissociated from membrane by high-salt buffers containing either 1.0 M NaCl or (NH4)2SO4 without detergents Citrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
cofactor NADP+, pH 7.0, 30°C Citrobacter sp.
0.47
-
cofactor NADP+, presence of CoA, pH 7.0, 30°C Citrobacter sp.
1.38
-
cofactor NAD+, pH 7.0, 30°C Citrobacter sp.
7.36
-
cofactor NAD+, presence of CoA, pH 7.0, 30°C Citrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
Citrobacter sp. acetyl-CoA + NADH
-
?
acetaldehyde + NAD+
-
Citrobacter sp. acetate + NADH + H+
-
?
butyraldehyde + CoA + NAD+ 21% of the activity with acetaldehyde Citrobacter sp. butanoyl-CoA + NADH
-
?
additional information bifunctional enzyme consisting of an N-terminal acetaldehyde dehydrogenase (ALDH) and a C-terminal alcohol dehydrogenase (ADH). The specificity constant (kcat/Km) is 47fold higher for acetaldehyde reductase than that for ethanol dehydrogenase Citrobacter sp. ?
-
?
propionaldehyde + CoA + NAD+ 51.8% of the activity with acetaldehyde Citrobacter sp. propanoyl-CoA + NADH
-
?
valeraldehyde + CoA + NAD+ 5.6% of the activity with acetaldehyde Citrobacter sp. valeryl-CoA + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 96000, SDS-PAGE Citrobacter sp.

Synonyms

Synonyms Comment Organism
AdhE
-
Citrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Citrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
inactivation above Citrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
acetaldehyde pH 7.0, 30°C Citrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Citrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
complete inactivation Citrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ 57fold preferred over NADP+ Citrobacter sp.
NADP+ NAD+ is 57fold preferred over NADP+ Citrobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
acetaldehyde pH 7.0, 30°C Citrobacter sp.