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Literature summary for 1.2.1.10 extracted from

  • Zhao, Y.; Lei, M.; Wu, Y.; Wang, C.; Zhang, Z.; Deng, F.; Wang, H.
    Molecular cloning and expression of the complete DNA sequence encoding NAD+-dependent acetaldehyde dehydrogenase from Acinetobacter sp. strain HBS-2 (2009), Ann. Microbiol., 59, 97-104.
No PubMed abstract available
Search for more literature for 1.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli BL21 cells Acinetobacter sp. HBS-2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00049
-
 propionaldehyde
-
 Acinetobacter sp. HBS-2
0.00212
-
 formaldehyde
-
 Acinetobacter sp. HBS-2
0.01518
-
 acetaldehyde
-
 Acinetobacter sp. HBS-2

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activity of the enzyme is elevated Acinetobacter sp. HBS-2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Acinetobacter sp. HBS-2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + CoA + NAD+ Acinetobacter sp. HBS-2
-
 acetyl-CoA + NADH + H+
-
 r
formaldehyde + CoA + NAD+ Acinetobacter sp. HBS-2
-
 formyl-CoA + NADH + H+
-
 ?
propionaldehyde + CoA + NAD+ Acinetobacter sp. HBS-2
-
 propionyl-CoA + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. HBS-2 A5JT11
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography by means of a Ni-Sepharose column Acinetobacter sp. HBS-2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.6
-
 recombinant enzyme Acinetobacter sp. HBS-2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Acinetobacter sp. HBS-2 acetyl-CoA + NADH + H+
-
 r
formaldehyde + CoA + NAD+
-
 Acinetobacter sp. HBS-2 formyl-CoA + NADH + H+
-
 ?
propionaldehyde + CoA + NAD+
-
 Acinetobacter sp. HBS-2 propionyl-CoA + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Acinetobacter sp. HBS-2

Synonyms

Synonyms Comment Organism
acetaldehyde dehydrogenase
-
 Acinetobacter sp. HBS-2
aldehyde dehydrogenase
-
 Acinetobacter sp. HBS-2
ALDH
-
 Acinetobacter sp. HBS-2

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Acinetobacter sp. HBS-2