Activating Compound | Comment | Organism | Structure |
---|---|---|---|
flavodoxin | cytochrome c reductase activity of FPR is stimulated three times by the addition of flavodoxin | Rhodobacter capsulatus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
ferricytochrome c | presence of flavodoxin, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus | |
0.08 | - |
NADPH | substrate ferricyanide, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Rhodobacter capsulatus |
30138 | - |
- |
Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | Q9L6V3 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH + H+ | - |
Rhodobacter capsulatus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricyanide + NADPH + H+ | - |
Rhodobacter capsulatus 37b4 | 2 ferrocyanide + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH + H+ | - |
Rhodobacter capsulatus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
additional information | enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen | Rhodobacter capsulatus | ? | - |
? | |
additional information | enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen | Rhodobacter capsulatus 37b4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE, 1 * 30138, calculated | Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
ferricytochrome c | presence of flavodoxin, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus | |
7.2 | - |
NADPH | substrate ferricyanide, pH 8.0, temperature not specified in the publication | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodobacter capsulatus |