Protein Variants | Comment | Organism |
---|---|---|
Y303F | about 30% of the wild-type enzyme activity with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin | Nostoc sp. |
Y303S | inactive. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered | Nostoc sp. |
Y303W | almost inactive | Nostoc sp. |
Y308F | about 20% of the wild-type enzyme activity with ferredoxin, about 11% of the wild-type enzyme activity with flavodoxin | Pisum sativum |
Y308S | nearly inactive mutant with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered | Pisum sativum |
Y308W | about 5% of the wild-type enzyme activity with ferredoxin, no activity with flavodoxin | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum | |
0.017 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
0.02 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
0.033 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
0.043 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Pisum sativum | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | P21890 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
Nostoc sp. ATCC 29151 | P21890 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
Pisum sativum | P10933 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced flavodoxin + NADP+ | - |
Pisum sativum | oxidized flavodoxin + NADPH + H+ | - |
? | |
reduced flavodoxin + NADP+ | - |
Nostoc sp. | oxidized flavodoxin + NADPH + H+ | - |
? | |
reduced flavodoxin + NADP+ | - |
Nostoc sp. ATCC 29151 | oxidized flavodoxin + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners | Pisum sativum |
More | the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners | Nostoc sp. |
Synonyms | Comment | Organism |
---|---|---|
PETH | - |
Pisum sativum |
PETH | - |
Nostoc sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
reduced flavodoxin | mutant Y303W, pH 8.0, 25°C | Nostoc sp. | |
4 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
7 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. | |
8.3 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
23.3 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
30.6 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. | |
200 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
500 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
700 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
1800 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum |