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Literature summary for 1.19.1.1 extracted from

  • Nogues, I.; Tejero, J.; Hurley, J.K.; Paladini, D.; Frago, S.; Tollin, G.; Mayhew, S.G.; Gomez-Moreno, C.; Ceccarelli, E.A.; Carrillo, N.; Medina, M.
    Role of the C-terminal tyrosine of ferredoxin-nicotinamide adenine dinucleotide phosphate reductase in the electron transfer processes with its protein partners ferredoxin and flavodoxin (2004), Biochemistry, 43, 6127-6137.
    View publication on PubMed
Search for more literature for 1.19.1.1

Protein Variants

Protein Variants Comment Organism
Y303F about 30% of the wild-type enzyme activity with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin Nostoc sp.
Y303S inactive. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered Nostoc sp.
Y303W almost inactive Nostoc sp.
Y308F about 20% of the wild-type enzyme activity with ferredoxin, about 11% of the wild-type enzyme activity with flavodoxin Pisum sativum
Y308S nearly inactive mutant with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered Pisum sativum
Y308W about 5% of the wild-type enzyme activity with ferredoxin, no activity with flavodoxin Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0167
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Pisum sativum
0.017
-
 reduced flavodoxin mutant Y308W, pH 8.0, 25°C Pisum sativum
0.02
-
 reduced flavodoxin mutant Y308F, pH 8.0, 25°C Pisum sativum
0.033
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Nostoc sp.
0.043
-
 reduced flavodoxin mutant Y303F, pH 8.0, 25°C Nostoc sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Pisum sativum 9507
-

Organism

Organism UniProt Comment Textmining
Nostoc sp. P21890 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-
Nostoc sp. ATCC 29151 P21890 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-
Pisum sativum P10933 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pisum sativum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced flavodoxin + NADP+
-
 Pisum sativum oxidized flavodoxin + NADPH + H+
-
 ?
reduced flavodoxin + NADP+
-
 Nostoc sp. oxidized flavodoxin + NADPH + H+
-
 ?
reduced flavodoxin + NADP+
-
 Nostoc sp. ATCC 29151 oxidized flavodoxin + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
More the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners Pisum sativum
More the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners Nostoc sp.

Synonyms

Synonyms Comment Organism
PETH
-
 Pisum sativum
PETH
-
 Nostoc sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 reduced flavodoxin mutant Y303W, pH 8.0, 25°C Nostoc sp.
4
-
 reduced flavodoxin mutant Y308F, pH 8.0, 25°C Pisum sativum
7
-
 reduced flavodoxin mutant Y303F, pH 8.0, 25°C Nostoc sp.
8.3
-
 reduced flavodoxin mutant Y308W, pH 8.0, 25°C Pisum sativum
23.3
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Nostoc sp.
30.6
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Pisum sativum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
170
-
 reduced flavodoxin mutant Y303F, pH 8.0, 25°C Nostoc sp.
200
-
 reduced flavodoxin mutant Y308F, pH 8.0, 25°C Pisum sativum
500
-
 reduced flavodoxin mutant Y308W, pH 8.0, 25°C Pisum sativum
700
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Nostoc sp.
1800
-
 reduced flavodoxin wild-type, pH 8.0, 25°C Pisum sativum