KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
0.0076 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMNH2 + NADP+ | Escherichia coli | - |
FMN + NADPH + H+ | - |
? | |
additional information | Escherichia coli | the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR | ? | - |
? | |
reduced flavodoxin I + NADP+ | Escherichia coli | - |
oxidized flavodoxin I + NADPH + H+ | - |
? | |
reduced flavodoxin II + NADP+ | Escherichia coli | - |
oxidized flavodoxin II + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P28861 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMNH2 + NADP+ | - |
Escherichia coli | FMN + NADPH + H+ | - |
? | |
additional information | the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR | Escherichia coli | ? | - |
? | |
reduced flavodoxin I + NADP+ | - |
Escherichia coli | oxidized flavodoxin I + NADPH + H+ | - |
? | |
reduced flavodoxin II + NADP+ | - |
Escherichia coli | oxidized flavodoxin II + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin (flavodoxin):NADP+ oxidoreductase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
0.0042 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
550 | - |
reduced flavodoxin I | pH 8.0, 25°C | Escherichia coli | |
980 | - |
reduced flavodoxin II | pH 8.0, 25°C | Escherichia coli | |
19000 | - |
FMNH2 | pH 8.0, 25°C | Escherichia coli |