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Literature summary for 1.18.6.2 extracted from

  • Dilworth, M.; Eldridge, M.; Eady, R.
    The molybdenum and vanadium nltrogenases of Azotobacter chroococcum Effect of elevated temperature on N2 reduction (1993), Biochem. J., 289, 395-400 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.6.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information over the temperature range 30-45 degrees C the apparent Km for the reduction of N2 is the same, but increases from 30 to 58 kPa of N2 Azotobacter chroococcum

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
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Azotobacter chroococcum MCD1 P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
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 Azotobacter chroococcum 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
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 ?
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
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 Azotobacter chroococcum MCD1 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
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 ?

Synonyms

Synonyms Comment Organism
vnfD alpha-chain Azotobacter chroococcum
vnfG delta-chain Azotobacter chroococcum
vnfK beta-chain Azotobacter chroococcum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 50 on increasing the temperature from 45 degrees C to 50 degrees C, little change occurrs in the rate of reduction of protons to H2. The rate of N2H4 production increases, but the rate of NH3 formation decreases 7fold. This selective loss of the ability to reduce N2 to NH3 is reversible Azotobacter chroococcum