Crystallization (Comment) | Organism |
---|---|
mutant enzyme R96Q bound to acetylene, capillary batch diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.1 M Tris (pH 8.0), 0.17 M Na2MoO4, and 0.001 M sodium dithionite | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
R96Q | the substitution of Arg to Gln at position 96 makes the active site pocket environment more hydrophobic than that of the native enzyme | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | P07328 and P07329 | alpha and beta chains | - |
Azotobacter vinelandii DJ1264 | P07328 and P07329 | alpha and beta chains | - |
Synonyms | Comment | Organism |
---|---|---|
nitrogenase MoFe protein | - |
Azotobacter vinelandii |
nitrogenase molybdenum-iron protein | - |
Azotobacter vinelandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-molybdenum cofactor | the enzyme contains 2 [8Fe-7S] P clusters and 2 active site [7Fe-9S-C-Mo-homocitrate] iron-molybdenum cofactors | Azotobacter vinelandii |