KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
3,3-difluorocyclopropene | pH 7.5, 30°C | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Azotobacter vinelandii OP | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3-difluorocyclopropene + dithionite | - |
Azotobacter vinelandii | propene + 2-fluoropropene + ? | major products providing evidence for reductive C-F bond cleavage. Synthesis of propene requires 6 e-/6 H+ and of 2-fluoropropene requires 4 e-/4 H+. In both products, C=C bond cleavage rather than C-C bond cleavage is involved. No selectivity is observed in formation of cis and trans isomers of 1,3-d2-2-fluoropropene, whereas cis-1,3-d2-propene is the predominant 1,3-d2-propene product, indicating that one of the bound reduction intermediates on the pathway to propene is constrained geometrically. Reduction requires both N2ase proteins MoFe and Fe protein, ATP, and an exogenous reductant such as dithionite. A reduction mechanism, consistent with hydride transfer as a key step, is discussed | ? | |
3,3-difluorocyclopropene + dithionite | - |
Azotobacter vinelandii OP | propene + 2-fluoropropene + ? | major products providing evidence for reductive C-F bond cleavage. Synthesis of propene requires 6 e-/6 H+ and of 2-fluoropropene requires 4 e-/4 H+. In both products, C=C bond cleavage rather than C-C bond cleavage is involved. No selectivity is observed in formation of cis and trans isomers of 1,3-d2-2-fluoropropene, whereas cis-1,3-d2-propene is the predominant 1,3-d2-propene product, indicating that one of the bound reduction intermediates on the pathway to propene is constrained geometrically. Reduction requires both N2ase proteins MoFe and Fe protein, ATP, and an exogenous reductant such as dithionite. A reduction mechanism, consistent with hydride transfer as a key step, is discussed | ? |