Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
General Information | Comment | Organism |
---|---|---|
metabolism | posttranslational regulation of nitrogenase activity in Rhodopseudomonas palustris depends on proteins DraT2, an ADP-ribosyltransferase, and GlnK2, an NtrC-regulated PII protein. GlnK2 is not well expressed in ammonium-grown NifA mutant cells that express nitrogenase genes constitutively and produce H2 when grown with ammonium as a nitrogen source. The mutant strain has elevated nitrogenase activity due to overexpression of the nif genes, and this increased amount of expression overwhelms a basal level of activity of DraT2 in ammonium-grown cells. Insufficient levels of both GlnK2 and DraT2 allow H2 production by an nifA* mutant grown with ammonium. Inactivation of the nitrogenase posttranslational modification system by mutation of draT2 results in increased H2 production by the ammonium-grown mutant cells | Rhodopseudomonas palustris |