BRENDA - Enzyme Database
show all sequences of 1.18.1.7

Metabolic engineering of Arabidopsis for remediation of different polycyclic aromatic hydrocarbons using a hybrid bacterial dioxygenase complex

Peng, R.; Fu, X.; Tian, Y.; Zhao, W.; Zhu, B.; Xu, J.; Wang, B.; Wang, L.; Yao, Q.; Metab. Eng. 26, 100-110 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Arabidopsis thaliana and Escherichia coli
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Pseudomonas putida
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Pseudomonas putida G7
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida G7
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
745688
Pseudomonas putida
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
745688
Pseudomonas putida G7
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
[2Fe-2S]-center
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Arabidopsis thaliana and Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
[2Fe-2S]-center
-
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Pseudomonas putida
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
reduced [2Fe-2S] ferredoxin + NADP+ + H+
Pseudomonas putida G7
-
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
745688
Pseudomonas putida
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
reduced [2Fe-2S] ferredoxin + NADP+ + H+
-
745688
Pseudomonas putida G7
oxidized [2Fe-2S] ferredoxin + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Pseudomonas putida
General Information
General Information
Commentary
Organism
physiological function
Arabidopsis plants overexpressing the heterozygous naphthalene dioxygenase system exhibit enhanced tolerance toward 2-4 rings polycyclic aromatic hydrocarbons
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Arabidopsis plants overexpressing the heterozygous naphthalene dioxygenase system exhibit enhanced tolerance toward 2-4 rings polycyclic aromatic hydrocarbons
Pseudomonas putida
Other publictions for EC 1.18.1.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745688
Peng
Metabolic engineering of Arab ...
Pseudomonas putida, Pseudomonas putida G7
Metab. Eng.
26
100-110
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
11
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722507
Zhou
Salicylate 5-hydroxylase from ...
Ralstonia sp., Ralstonia sp. U2
J. Bacteriol.
184
1547-1555
2002
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
437842
Haigler
Purification and properties of ...
Pseudomonas putida, Pseudomonas putida NCIB 9816
J. Bacteriol.
172
457-464
1990
-
-
-
-
-
-
5
-
-
1
3
-
-
3
-
-
1
-
-
-
1
1
10
1
-
-
1
-
-
-
-
2
-
1
1
-
-
-
2
-
-
-
1
5
-
-
-
1
3
-
-
-
-
1
-
-
1
1
10
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
722477
Haigler
Purification and properties of ...
Pseudomonas putida, Pseudomonas putida NCIB 9816
J. Bacteriol.
172
465-468
1990
-
-
-
-
-
-
-
-
-
1
1
-
-
3
-
-
1
-
-
-
4
-
4
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
4
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-