Cloned (Comment) | Organism |
---|---|
gene fprA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | transient and steady-state kinetics of diaphorase and flavin reductase activities, determination of reduction potential | Mycobacterium tuberculosis | |
0.0041 | - |
NADPH | diaphorase activity with ferricyanide, pH 7.2, 30°C | Mycobacterium tuberculosis | |
0.051 | - |
NADH | diaphorase activity with ferricyanide, pH 7.2, 30°C | Mycobacterium tuberculosis | |
0.16 | - |
ferricyanide | pH 7.2, 30°C | Mycobacterium tuberculosis | |
2.5 | - |
oxidized 2,6-dichloroindophenol | pH 7.2, 30°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49357 | - |
x * 49357, recombinant enzyme, mass spectrometry | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NAD(P)+ | Mycobacterium tuberculosis | - |
oxidized ferredoxin + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIQ3 | gene fprA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADPH | diaphorase activity | Mycobacterium tuberculosis | 2 ferrocyanide + NADP+ + H+ | - |
? | |
ferricyanide + NADH | - |
Mycobacterium tuberculosis | ferrocyanide + NAD+ + H+ | - |
r | |
additional information | the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site | Mycobacterium tuberculosis | ? | - |
? | |
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | diaphorase activity | Mycobacterium tuberculosis | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADPH | - |
Mycobacterium tuberculosis | reduced 2,6-dichlorophenolindophenol + NADP+ + H+ | - |
r | |
reduced ferredoxin + NAD(P)+ | - |
Mycobacterium tuberculosis | oxidized ferredoxin + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 49357, recombinant enzyme, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin reductase | - |
Mycobacterium tuberculosis |
FprA | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
oxidized 2,6-dichlorophenolindophenol | pH 7.2, 30°C | Mycobacterium tuberculosis | |
25.6 | - |
NADH | flavin reduction, pH 7.2, 30°C | Mycobacterium tuberculosis | |
33.7 | - |
ferricyanide | pH 7.2, 30°C | Mycobacterium tuberculosis | |
50.6 | - |
NADPH | flavin reduction, pH 7.2, 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme, determination of reduction potential | Mycobacterium tuberculosis | |
additional information | the enzyme contains a second, kinetically distinct and inhibitory pyridine nucleotide-binding site | Mycobacterium tuberculosis | |
NAD(P)+ | preference for NADP+ | Mycobacterium tuberculosis |