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Literature summary for 1.18.1.4 extracted from

  • Cabeza, M.; Guerrero, S.; Iglesias, A.; Arias, D.
    New enzymatic pathways for the reduction of reactive oxygen species in Entamoeba histolytica (2015), Biochim. Biophys. Acta, 1850, 1233-1244 .
    View publication on PubMed
Search for more literature for 1.18.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
additional information no substrate inhibition at high NAD(P)H (up to 0.3 mM) Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
0.0032
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
0.0043
-
 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
0.0052
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel filtration Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica HM1-IMSS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap IMAC-Ni2+ column chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins Entamoeba histolytica ?
-
 ?
additional information the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins Entamoeba histolytica HM1-IMSS ?
-
 ?
oxidized Clostridium pasteurianum rubredoxin + NADH
-
 Entamoeba histolytica reduced Clostridium pasteurianum rubredoxin + NAD+ + H+
-
 ?
oxidized Clostridium pasteurianum rubredoxin + NADH
-
 Entamoeba histolytica HM1-IMSS reduced Clostridium pasteurianum rubredoxin + NAD+ + H+
-
 ?
oxidized Clostridium pasteurianum rubredoxin + NADPH
-
 Entamoeba histolytica reduced Clostridium pasteurianum rubredoxin + NADP+ + H+
-
 ?
oxidized Clostridium pasteurianum rubredoxin + NADPH
-
 Entamoeba histolytica HM1-IMSS reduced Clostridium pasteurianum rubredoxin + NADP+ + H+
-
 ?
oxidized flavodiiron protein + NADH
-
 Entamoeba histolytica reduced flavodiiron protein + NAD+ + H+
-
 ?
oxidized flavodiiron protein + NADH
-
 Entamoeba histolytica HM1-IMSS reduced flavodiiron protein + NAD+ + H+
-
 ?
oxidized flavodiiron protein + NADPH
-
 Entamoeba histolytica reduced flavodiiron protein + NADP+ + H+
-
 ?
oxidized flavodiiron protein + NADPH
-
 Entamoeba histolytica HM1-IMSS reduced flavodiiron protein + NADP+ + H+
-
 ?
oxidized Pyrococcus furiosus rubredoxin + NADH
-
 Entamoeba histolytica reduced Pyrococcus furiosus rubredoxin + NAD+ + H+
-
 ?
oxidized Pyrococcus furiosus rubredoxin + NADPH
-
 Entamoeba histolytica reduced Pyrococcus furiosus rubredoxin + NADP+ + H+
-
 ?
oxidized rubrerythrin + NADH
-
 Entamoeba histolytica reduced rubrerythrin + NAD+ + H+
-
 ?
oxidized rubrerythrin + NADPH
-
 Entamoeba histolytica reduced rubrerythrin + NADP+ + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Entamoeba histolytica
monomer 1 * 49700, calculated from amino acid sequence Entamoeba histolytica

Synonyms

Synonyms Comment Organism
NAD(P)H-dependent rubredoxin reductase
-
 Entamoeba histolytica
NROR
-
 Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Entamoeba histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
9.6
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
13.9
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
69.4
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Entamoeba histolytica
NADH the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH Entamoeba histolytica
NADPH the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica calculated from amino acid sequence
-
 8.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3000
-
 oxidized Pyrococcus furiosus rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
5000
-
 oxidized Clostridium pasteurianum rubredoxin with NADPH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
8400
-
 oxidized Clostridium pasteurianum rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica
13000
-
 oxidized Pyrococcus furiosus rubredoxin with NADH as cosubstrate, at pH 7.0 and 30°C Entamoeba histolytica