Literature summary for 1.18.1.4 extracted from

Grunden, A.M.; Jenney, F.E.; Ma, K.; Ji, M.; Weinberg, M.V.; Adams, M.W.
In vitro reconstitution of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus (2005), Appl. Environ. Microbiol., 71, 1522-1530.

Search for more literature for 1.18.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus furiosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	oxidized rubredoxin	pH 8, 23°C, cosubstrate: NADPH	Pyrococcus furiosus
0.037	-	oxidized rubredoxin	pH 8, 80°C, cosubstrate: NADPH	Pyrococcus furiosus
0.048	-	NADPH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
0.18	-	NADH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
5.5	-	Oxidized benzyl viologen	pH 8, 80°C, cosubstrate: NADH	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39900	-	x * 39900, calculated from sequence	Pyrococcus furiosus
40000	-	x * 40000, SDS-PAGE	Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 oxidized rubredoxin + NADPH	Pyrococcus furiosus	superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase	2 reduced rubredoxin + NADP+ + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q8U1K9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 oxidized rubredoxin + NADPH	-	Pyrococcus furiosus	2 reduced rubredoxin + NADP+ + H+	-	?
2 oxidized rubredoxin + NADPH	superoxide reductase mediates reduction of superoxide to hydrogen peroxide in an NADPH-dependent manner via a coupled reaction between NAD(P)H:rubredoxin oxidoreductase, rubredoxin, and superoxide reductase	Pyrococcus furiosus	2 reduced rubredoxin + NADP+ + H+	-	?
oxidized benzyl viologen + NADH	-	Pyrococcus furiosus	reduced benzyl viologen + NAD+ + H+	-	?
oxidized benzyl viologen + NADPH	-	Pyrococcus furiosus	reduced benzyl viologen + NADP+ + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Pyrococcus furiosus
?	x * 39900, calculated from sequence	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
NROR	-	Pyrococcus furiosus
PF1197	-	Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
258	-	NADPH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
350	-	oxidized rubredoxin	pH 8, 23°C, cosubstrate: NADPH	Pyrococcus furiosus
498	-	NADH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
1236	-	Oxidized benzyl viologen	pH 8, 80°C, cosubstrate: NADH	Pyrococcus furiosus
8980	-	oxidized rubredoxin	pH 8, 80°C, cosubstrate: NADPH	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Pyrococcus furiosus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	essential for activity, can not be replaced by FMN	Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
225	-	Oxidized benzyl viologen	pH 8, 80°C, cosubstrate: NADH	Pyrococcus furiosus
2770	-	NADH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
5375	-	NADPH	pH 8, 80°C, cosubstrate: benzyl viologen	Pyrococcus furiosus
218900	-	oxidized rubredoxin	pH 8, 23°C, cosubstrate: NADPH	Pyrococcus furiosus
242700	-	oxidized rubredoxin	pH 8, 80°C, cosubstrate: NADPH	Pyrococcus furiosus

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Release 2023.1 (January 2023)