Purification and characterization of the NADH:ferredoxinBPH oxidoreductase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400

Broadus, R.M.; Haddock, J.D.; Arch. Microbiol. 170, 106-112 (1998)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.058

-

NADH

reaction with 2,6-dichlorophenolindophenol

Pseudomonas sp.

0.156

-

NADPH

reaction with 2,6-dichlorophenolindophenol

Pseudomonas sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2 ferricyanide + NADH

-

285290

Pseudomonas sp.

2 ferrocyanide + NAD+ + H+

-

-

-

?

2 ferricyanide + NADH

-

285290

Pseudomonas sp. LB400

2 ferrocyanide + NAD+ + H+

-

-

-

?

2 ferricytochrome c + NADH

-

285290

Pseudomonas sp.

2 ferrocytochrome c + NAD+ + H+

-

-

-

?

2 ferricytochrome c + NADH

-

285290

Pseudomonas sp. LB400

2 ferrocytochrome c + NAD+ + H+

-

-

-

?

NADH + oxidized 2,6-dichlorophenolindophenol + H+

-

285290

Pseudomonas sp.

NAD+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADH + oxidized 2,6-dichlorophenolindophenol + H+

-

285290

Pseudomonas sp. LB400

NAD+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADH + oxidized nitro blue tetrazolium + H+

-

285290

Pseudomonas sp.

NAD+ + reduced nitro blue tetrazolium

-

-

-

?

NADPH + H+ + oxidized 2,6-dichlorophenolindophenol

-

285290

Pseudomonas sp.

NADP+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADPH + H+ + oxidized 2,6-dichlorophenolindophenol

-

285290

Pseudomonas sp. LB400

NADP+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

Cofactor

Commentary

Organism

Structure

FAD

contains 0.89 mol of FAD per mol of enzyme

Pseudomonas sp.

NADH

-

Pseudomonas sp.

NADPH

-

Pseudomonas sp.

Cofactor

Commentary

Organism

Structure

FAD

contains 0.89 mol of FAD per mol of enzyme

Pseudomonas sp.

NADH

-

Pseudomonas sp.

NADPH

-

Pseudomonas sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.058

-

NADH

reaction with 2,6-dichlorophenolindophenol

Pseudomonas sp.

0.156

-

NADPH

reaction with 2,6-dichlorophenolindophenol

Pseudomonas sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2 ferricyanide + NADH

-

285290

Pseudomonas sp.

2 ferrocyanide + NAD+ + H+

-

-

-

?

2 ferricyanide + NADH

-

285290

Pseudomonas sp. LB400

2 ferrocyanide + NAD+ + H+

-

-

-

?

2 ferricytochrome c + NADH

-

285290

Pseudomonas sp.

2 ferrocytochrome c + NAD+ + H+

-

-

-

?

2 ferricytochrome c + NADH

-

285290

Pseudomonas sp. LB400

2 ferrocytochrome c + NAD+ + H+

-

-

-

?

NADH + oxidized 2,6-dichlorophenolindophenol + H+

-

285290

Pseudomonas sp.

NAD+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADH + oxidized 2,6-dichlorophenolindophenol + H+

-

285290

Pseudomonas sp. LB400

NAD+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADH + oxidized nitro blue tetrazolium + H+

-

285290

Pseudomonas sp.

NAD+ + reduced nitro blue tetrazolium

-

-

-

?

NADPH + H+ + oxidized 2,6-dichlorophenolindophenol

-

285290

Pseudomonas sp.

NADP+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

NADPH + H+ + oxidized 2,6-dichlorophenolindophenol

-

285290

Pseudomonas sp. LB400

NADP+ + reduced 2,6-dichlorophenolindophenol

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

746345

Ke

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Characterization of a novel C ...

Acinetobacter sp., Nocardia farcinica

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Reduction of flavodoxin by el ...

Acidaminococcus fermentans, Acidaminococcus fermentans DSM 20731

J. Biol. Chem.

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11993-12002

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Wang

The role of Rnf in ion gradie ...

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1

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744269

Nishizawa

Complete pyridine-nucleotide- ...

Acidovorax sp. KKS102

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1

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727962

Hess

The ferredoxin: NAD+ oxidoredu ...

Acetobacterium woodii, Acetobacterium woodii DSM 103

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728235

Tremblay

The Rnf complex of Clostridium ...

Clostridium ljungdahlii, Clostridium ljungdahlii DSM 13528

mBio

4

e00406

2012

-

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746333

Dutta

-

Purification and properties o ...

Pseudomonas chlororaphis subsp. aureofaciens

Process Biochem.

47

1263-1267

2012

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1

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1

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-

706994

Umeda

Crystallization and preliminar ...

Novosphingobium sp., Novosphingobium sp. KA1

Acta Crystallogr. Sect. F

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712-714

2010

-

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1

1

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684177

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Crystallization and preliminar ...

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684179

Senda

Crystallization and preliminar ...

Acidovorax sp.

Acta Crystallogr. Sect. F

63

520-523

2007

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1

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658218

Zanno

MT FdR: a ferredoxin reductase ...

Mycobacterium tuberculosis

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157-169

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673520

Sielaff

Kinetic and binding studies wi ...

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8

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1

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675830

Sielaff

Analysis of the nearly identic ...

Mycobacterium sp., Mycobacterium sp. HE5

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2593-2603

2005

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658575

Hussain

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Ferredoxin reductase enhances ...

Streptomyces coelicolor

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790-800

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Senda

Crystal structure of NADH-depe ...

Pseudomonas sp.

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Shen

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Biochemical basis for carbon m ...

[Butyribacterium] methylotrophicum

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285290

Broadus

Purification and characterizat ...

Pseudomonas sp., Pseudomonas sp. LB400

Arch. Microbiol.

170

106-112

1998

-

-

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-

-

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2

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2

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12

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1

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9

1

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3

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Shaw

Purification and characterisa ...

Pseudomonas putida

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51-61

1992

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Primary structure of xylene m ...

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Haigler

Purification and properties of ...

Pseudomonas sp., Pseudomonas sp. NCIB 9816

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457-464

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1

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10

1

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Isolation, characterization, a ...

Methylosinus trichosporium OB3b

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5012-5016

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Ethanol production by thermoph ...

Thermoanaerobacter thermohydrosulfuricus

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437846

Subramanian

Purification and properties of ...

Pseudomonas putida

J. Biol. Chem.

256

2723-2730

1981

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3

2

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1

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6

1

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437847

Blusson

A new, fast, and sensitive ass ...

Clostridium pasteurianum, Clostridium tyrobutyricum, Clostridium tyrobutyricum CNRZ 510

Anal. Biochem.

110

176-181

1981

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3

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437848

Lamed

Ethanol production by thermoph ...

Thermoanaerobacter brockii, Hungateiclostridium thermocellum

J. Bacteriol.

144

569-578

1980

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437849

Petitdemange

Study of the NADH and NADPH-fe ...

Clostridium acetobutylicum

Can. J. Microbiol.

23

152-160

1977

1

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437850

Petitdemange

Regulation of the NADH and NAD ...

Clostridium acetobutylicum, Clostridium pasteurianum, Clostridium tyrobutyricum

Biochim. Biophys. Acta

421

334-347

1976

3

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7

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437851

Jungermann

Function of reduced pyridine n ...

Clostridium butyricum, Clostridium pasteurianum

Biochim. Biophys. Acta

305

268-280

1973

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437843

Jungermann

Demonstration of NADH-ferredox ...

Clostridium butyricum, Clostridium kluyveri, Clostridium pasteurianum

Arch. Mikrobiol.

80

370-372

1971

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