Protein Variants | Comment | Organism |
---|---|---|
additional information | deletions of Tyr326-Glu360 decrease the hydride transfer rate and the amount of reduced enzyme increases at equilibrium relative to wild type. Deletions of Phe337-Glu360 and Ser338-Glu360 result in slight changes in the reaction kinetics and redox equilibrium compared to the wild type enzyme | Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0142 | - |
NADPH | with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C | Chlorobaculum tepidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
gel filtration | Chlorobaculum tepidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced ferredoxin + NADP+ + H+ | Chlorobaculum tepidum | - |
oxidized ferredoxin + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferricyanide + NADPH | - |
Chlorobaculum tepidum | ferrocyanide + NADP+ + H+ | - |
r | |
reduced ferredoxin + NADP+ + H+ | - |
Chlorobaculum tepidum | oxidized ferredoxin + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Chlorobaculum tepidum |
? | x * 39190, MALDI-TOF mass spectrometry | Chlorobaculum tepidum |
? | x * 39240, calculated from amino acid sequence | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NAD(P)+ reductase | - |
Chlorobaculum tepidum |
FNR | - |
Chlorobaculum tepidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
213 | - |
NADPH | with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C | Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Chlorobaculum tepidum | |
NADPH | - |
Chlorobaculum tepidum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15000 | - |
NADPH | with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C | Chlorobaculum tepidum |