Literature summary for 1.18.1.2 extracted from

Komori, H.; Seo, D.; Sakurai, T.; Higuchi, Y.
Crystallization and preliminary X-ray studies of ferredoxin-NADP+ oxidoreductase encoded by Bacillus subtilis yumC (2010), Acta Crystallogr. Sect. F, 66, 301-303.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant FNR in complex with NADP+ in two different forms, 0.001 ml of 10 mg/ml protein in 50 mM Tris-HCl pH 8.0, 200 mM NaCl, and 2.5 mM NADP+, is mixed with 0.001 ml reservoir solution, 20°C. The reservoir solutions consist of 0.1 M HEPES buffer pH 7.5, 30% 1,2-propanediol, 20% PEG 400 for form I, and of 20% PEG 3350, 0.2 M sodium fluoride and 5% trehalose for form II. X-ray diffraction structure determination and analysis at 1.8 and 1.9 A resolution, respectively, molecular replacement, modelling	Bacillus subtilis

Crystallization (Comment)

Organism

purified recombinant FNR in complex with NADP+ in two different forms, 0.001 ml of 10 mg/ml protein in 50 mM Tris-HCl pH 8.0, 200 mM NaCl, and 2.5 mM NADP+, is mixed with 0.001 ml reservoir solution, 20°C. The reservoir solutions consist of 0.1 M HEPES buffer pH 7.5, 30% 1,2-propanediol, 20% PEG 400 for form I, and of 20% PEG 3350, 0.2 M sodium fluoride and 5% trehalose for form II. X-ray diffraction structure determination and analysis at 1.8 and 1.9 A resolution, respectively, molecular replacement, modelling

Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	gene yumC	-

Synonyms

Synonyms	Comment	Organism
ferredoxin-NADP+ oxidoreductase	-	Bacillus subtilis
FNR	-	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	noncovalently bound	Bacillus subtilis

General Information

General Information	Comment	Organism
physiological function	FNR mediates the redox reaction between NADP+/NADPH and ferredoxin, providing redox equivalents in cell-material synthesis	Bacillus subtilis

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Release 2023.1 (January 2023)