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Literature summary for 1.18.1.2 extracted from
- In vitro and in vivo interactions of ferredoxin-NADP+ reductases in Pseudomonas putida (2009), J. Biochem., 145, 481-491.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed via plasmid in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00083 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida |  |
| 0.00111 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 0.00112 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 0.00181 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 0.00191 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 0.00191 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 0.00245 | - |
NADPH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 0.00263 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 0.00298 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 0.00326 | - |
NADPH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 0.00397 | - |
NADPH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 0.00464 | - |
NADH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 0.00479 | - |
NADPH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 0.00615 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 0.00686 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 0.00717 | - |
NADH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 0.00802 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 0.00822 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 0.00933 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 0.00943 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 0.01063 | - |
NADH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 0.01163 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 0.02363 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 0.0632 | - |
NADH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [4Fe-4S]-ferredoxin + NADPH | Pseudomonas putida | [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) | reduced [4Fe-4S]-ferredoxin + NADP+ | - |
r | |
| [4Fe-4S]-ferredoxin + NADPH | Pseudomonas putida KT 2240 | [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) | reduced [4Fe-4S]-ferredoxin + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
wild type and mutants fprA (lacking FprA) and fprB (lacking FprB) | - |
| Pseudomonas putida KT 2240 | - |
wild type and mutants fprA (lacking FprA) and fprB (lacking FprB) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + K3Fe(CN)6 | - |
Pseudomonas putida | NAD+ + reduced K3Fe(CN)6 | - |
r | |
| NADH + K3Fe(CN)6 | - |
Pseudomonas putida KT 2240 | NAD+ + reduced K3Fe(CN)6 | - |
r | |
| NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida KT 2240 | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| NADPH + K3Fe(CN)6 | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | NADP+ + reduced K3Fe(CN)6 | - |
r | |
| NADPH + K3Fe(CN)6 | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida KT 2240 | NADP+ + reduced K3Fe(CN)6 | - |
r | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Pseudomonas putida | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Pseudomonas putida KT 2240 | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| oxidized ferredoxin + NADH + cytochrome c | - |
Pseudomonas putida | reduced ferredoxin + NAD+ + reduced cytochrome c | - |
r | |
| oxidized ferredoxin + NADPH + cytochrome c | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | reduced ferredoxin + NADP+ + reduced cytochrome c | - |
r | |
| [4Fe-4S]-ferredoxin + NADPH | [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) | Pseudomonas putida | reduced [4Fe-4S]-ferredoxin + NADP+ | - |
r | |
| [4Fe-4S]-ferredoxin + NADPH | [4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld) | Pseudomonas putida KT 2240 | reduced [4Fe-4S]-ferredoxin + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, fast protein liquid chromatography | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferredoxin-NADP+ reductase | - |
Pseudomonas putida |
| FPR | - |
Pseudomonas putida |
| FprA | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
| FprB | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.16 | - |
NADH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 3.03 | - |
NADH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 3.09 | - |
NADH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 3.82 | - |
NADPH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 4.51 | - |
NADPH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 4.59 | - |
NADH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 4.77 | - |
NADPH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 5.99 | - |
NADPH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 90 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 90 | - |
NADH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 110 | - |
NADH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 130 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 170 | - |
NADH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 530 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 540 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 680 | - |
NADH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 770 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor | Pseudomonas putida | |
| 860 | - |
NADH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 1000 | - |
NADPH | for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 1130 | - |
NADPH | for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 1140 | - |
NADPH | for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor | Pseudomonas putida | |
| 1370 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 1860 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 3050 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 3230 | - |
NADPH | for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor | Pseudomonas putida | |
| 3720 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
| 4590 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 4630 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor | Pseudomonas putida | |
| 6730 | - |
NADH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 11000 | - |
NADPH | for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor | Pseudomonas putida | |
| 11510 | - |
NADPH | for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor | Pseudomonas putida | |
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