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Literature summary for 1.18.1.2 extracted from
- Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase (2002), Eur. J. Biochem., 269, 3005-3013.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fprA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Mycobacterium tuberculosis | |
| 0.00045 | - |
NADPH | recombinant enzyme, pH 8.2, 25°C, with Fe(CN)63- | Mycobacterium tuberculosis |  |
| 0.00089 | - |
NADPH | recombinant enzyme, pH 8.2, 25°C, with 2,6-dichlorophenolindophenol | Mycobacterium tuberculosis | |
| 0.015 | - |
Fe(CN)63- | recombinant enzyme, pH 8.2, 25°C, with NADH | Mycobacterium tuberculosis | |
| 0.022 | - |
Fe(CN)63- | recombinant enzyme, pH 8.2, 25°C, with NADPH | Mycobacterium tuberculosis | |
| 0.056 | - |
reduced 2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with NADH | Mycobacterium tuberculosis | |
| 0.058 | - |
reduced 2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with NADPH | Mycobacterium tuberculosis | |
| 0.068 | - |
oxidized 2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with Fe(CN)63- | Mycobacterium tuberculosis | |
| 0.083 | - |
oxidized 2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with 2,6-dichlorophenolindophenol | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
1 * 50000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
| 53000 | - |
recombinant enzyme, gel filtration | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene fprA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, gel filtration, and anion-exchange chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NAD(P)H | high diaphorase activity | Mycobacterium tuberculosis | 2 ferrocyanide + NAD(P)+ + H+ | - |
? | |
| 2,6-dichlorophenolindophenol + NADPH + H+ | - |
Mycobacterium tuberculosis | reduced 2,6-dichlorophenolindophenol + NADP+ | - |
r | |
| NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | high diaphorase activity | Mycobacterium tuberculosis | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH + H+ | - |
Mycobacterium tuberculosis | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
| reduced 2,6-dichlorophenolindophenol + NADP+ | - |
Mycobacterium tuberculosis | oxidized 2,6-dichlorophenolindophenol + NADPH + H+ | - |
r | |
| reduced ferredoxin + NADP+ | low activity | Mycobacterium tuberculosis | oxidized ferredoxin + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
| More | enzyme forms aggregates in solution which can be prevented by addition of 10% glycerol and 2-mercaptoethanol | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FprA | - |
Mycobacterium tuberculosis |
| NADPH ferredoxin reductase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21 | - |
2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with NADH | Mycobacterium tuberculosis | |
| 25.6 | - |
2,6-dichlorophenolindophenol | recombinant enzyme, pH 8.2, 25°C, with NADPH | Mycobacterium tuberculosis | |
| 42 | - |
Fe(CN)63- | recombinant enzyme, pH 8.2, 25°C, with NADH | Mycobacterium tuberculosis | |
| 63 | - |
Fe(CN)63- | recombinant enzyme, pH 8.2, 25°C, with NADPH | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, ferric reductase activity | Mycobacterium tuberculosis |
| 8.2 | - |
assay at, diaphorase activity | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 0.98 mol FAD tightly bound to 1 mol of enzyme | Mycobacterium tuberculosis | |
| NAD+ | diaphorase activity, low activity with | Mycobacterium tuberculosis | |
| NADH | diaphorase activity, low activity with | Mycobacterium tuberculosis | |
| NADP+ | - |
Mycobacterium tuberculosis | |
| NADPH | - |
Mycobacterium tuberculosis | |
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