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Literature summary for 1.18.1.2 extracted from

  • Seo, D.; Kamino, K.; Inoue, K.; Sakurai, H.
    Purification and characterization of ferredoxin-NADP+ reductase encoded by Bacillus subtilis yumC (2004), Arch. Microbiol., 182, 80-89.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information below 0.1 mM, oxidized ferredoxin with NADH, pH 7.0, 24°C Bacillus subtilis
0.54
-
oxidized ferredoxin with NADPH, pH 7.0, 24°C Bacillus subtilis
0.57
-
NADPH 2,6-dichlorophenolindophenol reduction, pH 7.0, 24°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Bacillus subtilis
94000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced ferredoxin + NADP+ Bacillus subtilis
-
oxidized ferredoxin + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene yumC
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from cell extract 86fold to homogeneity by several chromatographic steps Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.8
-
purified enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also shows NAD(P)H oxidase activity, overview Bacillus subtilis ?
-
?
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity, no activity with NADH Bacillus subtilis NADP+ + reduced 2,6-dichlorophenolindophenol
-
?
oxidized ferredoxin + NADH + H+
-
Bacillus subtilis reduced ferredoxin + NAD+
-
r
reduced ferredoxin + NADP+
-
Bacillus subtilis oxidized ferredoxin + NADPH
-
?
reduced ferredoxin + NADP+
-
Bacillus subtilis oxidized ferredoxin + NADPH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
FNR
-
Bacillus subtilis
YumC
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
NADH ferredoxin reduction, pH 7.0, 24°C Bacillus subtilis
22.7
-
NADPH 2,6-dichlorophenolindophenol reduction, pH 7.0, 24°C Bacillus subtilis
35.4
-
NADPH ferredoxin reduction, pH 7.0, 24°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Bacillus subtilis
NADP+
-
Bacillus subtilis
NADPH reverse reaction: preferred cofactor, poor activity with NADH Bacillus subtilis