Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modelling, overview | Clostridium acetobutylicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | Q9AL95 | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | NROR exists as a monomer in solution, the overall structure of NROR displays a GR-fold | Clostridium acetobutylicum |
Synonyms | Comment | Organism |
---|---|---|
NADH:rubredoxin oxidoreductase | - |
Clostridium acetobutylicum |
NROR | - |
Clostridium acetobutylicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding structure, overview | Clostridium acetobutylicum | |
NADH | binding structure, overview | Clostridium acetobutylicum |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme's disulfide bonds are not involved in the electron transfer from NADH to rubredoxin catalyzed by NROR | Clostridium acetobutylicum |