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Literature summary for 1.17.99.6 extracted from

  • Miles, Z.D.; McCarty, R.M.; Molnar, G.; Bandarian, V.
    Discovery of epoxyqueuosine (oQ) reductase reveals parallels between halorespiration and tRNA modification (2011), Proc. Natl. Acad. Sci. USA, 108, 7368-7372.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Cobalamin stimulates Escherichia coli
Cobalamin stimulates Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
epoxyqueuosine34 in tRNA + reduced acceptor Escherichia coli the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn queuosine34 in tRNA + acceptor + H2O
-
?
epoxyqueuosine34 in tRNA + reduced acceptor Bacillus subtilis the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn queuosine34 in tRNA + acceptor + H2O
-
?
epoxyqueuosine34 in tRNA + reduced acceptor Bacillus subtilis 168 the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn queuosine34 in tRNA + acceptor + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P97030
-
-
Bacillus subtilis 168 P97030
-
-
Escherichia coli P39288
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxyqueuosine34 in tRNA + reduced acceptor the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn Escherichia coli queuosine34 in tRNA + acceptor + H2O
-
?
epoxyqueuosine34 in tRNA + reduced acceptor the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn Bacillus subtilis queuosine34 in tRNA + acceptor + H2O
-
?
epoxyqueuosine34 in tRNA + reduced acceptor the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn Bacillus subtilis 168 queuosine34 in tRNA + acceptor + H2O
-
?
epoxyqueuosine34 in tRNA + reduced methyl viologen conversion of epoxyqueuosine to queuoseine is carried out using either a 17-mer oQ-containing RNA stem loop Escherichia coli queuosine34 in tRNA + methyl viologen + H2O
-
?
epoxyqueuosine34 in tRNA + reduced methyl viologen the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain Bacillus subtilis queuosine34 in tRNA + methyl viologen + H2O
-
?
epoxyqueuosine34 in tRNA + reduced methyl viologen the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain Bacillus subtilis 168 queuosine34 in tRNA + methyl viologen + H2O
-
?

Synonyms

Synonyms Comment Organism
oQ reductase
-
Escherichia coli
oQ reductase
-
Bacillus subtilis
QueG
-
Escherichia coli
QueG
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Escherichia coli
7.2
-
assay at Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn Escherichia coli
metabolism the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn Bacillus subtilis