KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0098 | - |
ferrocenium | with ethylbenzene as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.018 | - |
ferrocenium | with 3-phenyl-1-propene as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.028 | - |
ferricyanide | with ethylbenzene as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.035 | - |
ferrocenium | with 3-phenyl-1-propyne as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.362 | - |
ferricyanide | with 3-phenyl-1-propene as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.77 | - |
ferricyanide | with 3-phenyl-1-propyne as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Aromatoleum aromaticum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylbenzene + H2O + acceptor | Aromatoleum aromaticum | - |
(S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + acceptor | Aromatoleum aromaticum EbN1 | - |
(S)-1-phenylethanol + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum aromaticum | - |
- |
- |
Aromatoleum aromaticum EbN1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography | Aromatoleum aromaticum |
Storage Stability | Organism |
---|---|
4°C, completely purified enzyme, 24 days, 78% loss of activity | Aromatoleum aromaticum |
4°C, partially purified enzyme, 6 days, almost no loss of activity | Aromatoleum aromaticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ethylthiophene + ferricyanide | - |
Aromatoleum aromaticum | (S)-1-(2-thienyl)-ethanol + ferrocyanide | - |
? | |
2-ethylthiophene + ferricyanide | - |
Aromatoleum aromaticum EbN1 | (S)-1-(2-thienyl)-ethanol + ferrocyanide | - |
? | |
3-phenyl-1-propene + H2O + ferricyanide | - |
Aromatoleum aromaticum | ? + ferrocyanide | - |
? | |
3-phenyl-1-propene + H2O + ferricyanide | - |
Aromatoleum aromaticum EbN1 | ? + ferrocyanide | - |
? | |
3-phenyl-1-propene + H2O + ferrocenium | - |
Aromatoleum aromaticum | ? + ferrocene | - |
? | |
3-phenyl-1-propene + H2O + ferrocenium | - |
Aromatoleum aromaticum EbN1 | ? + ferrocene | - |
? | |
3-phenyl-1-propyne + H2O + ferricyanide | - |
Aromatoleum aromaticum | ? + ferrocyanide | - |
? | |
3-phenyl-1-propyne + H2O + ferricyanide | - |
Aromatoleum aromaticum EbN1 | ? + ferrocyanide | - |
? | |
3-phenyl-1-propyne + H2O + ferrocenium | - |
Aromatoleum aromaticum | ? + ferrocene | - |
? | |
ethylbenzene + H2O + acceptor | - |
Aromatoleum aromaticum | (S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + acceptor | - |
Aromatoleum aromaticum EbN1 | (S)-1-phenylethanol + reduced acceptor | - |
? | |
ethylbenzene + H2O + ferricyanide | - |
Aromatoleum aromaticum | (S)-1-phenylethanol + ferrocyanide | - |
? | |
ethylbenzene + H2O + ferrocenium | - |
Aromatoleum aromaticum | (S)-1-phenylethanol + ferrocene | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EBDH | - |
Aromatoleum aromaticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
ethylbenzene | with ferricyanide as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.27 | - |
ethylbenzene | with ferrocenium as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.29 | - |
3-phenyl-1-propyne | with ferrocenium as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.33 | - |
3-phenyl-1-propyne | with ferricyanide as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.46 | - |
3-phenyl-1-propene | with ferrocenium as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum | |
0.64 | - |
3-phenyl-1-propene | with ferricyanide as cosubstrate, at pH 7.5 and 25°C | Aromatoleum aromaticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
bis(molybdopterin guanine dinucleotide)molybdenum | - |
Aromatoleum aromaticum | |
heme | - |
Aromatoleum aromaticum | |
iron-sulfur centre | - |
Aromatoleum aromaticum |