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Literature summary for 1.17.98.3 extracted from
- Properties of formate dehydrogenase in Methanobacterium formicicum (1982), J. Bacteriol., 150, 1-7.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM, 33% inhibition | Methanobacterium formicicum |  |
| 2,2'-dipyridyl | 5 mM, 87% inhibition | Methanobacterium formicicum | |
| azide | - |
Methanobacterium formicicum | |
| cyanide | - |
Methanobacterium formicicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
oxidized coenzyme F420 | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
| 0.013 | - |
FMN | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
| 0.025 | - |
FAD | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | the purified enzyme contains 0.0074 mM of molybdenum per g of protein | Methanobacterium formicicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 288000 | - |
gel filtration | Methanobacterium formicicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacterium formicicum | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the enzyme is extremely sensitive to oxygen. The exposure of crude cell-free extract to 101.3 kPa (1 atm) of air results in rapid loss of activity. The removal of air by vacuum degassing with O2-free N2 and the addition of 1 mM sodium dithionite does not restore activity.Sodium azide (10 mM) partially protected the against inactivation by air | Methanobacterium formicicum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanobacterium formicicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0009 | - |
substrate: FMN, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
| 0.0016 | - |
substrate: FAD, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
| 0.0082 | - |
substrate: oxidized coenzyme F420, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
| 0.059 | - |
substrate: oxidized methyl viologen, pH 7.5, temperature not specified in the publication | Methanobacterium formicicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + FAD | specific activity 5.4fold lower than for the reaction with coenzyme F420. Vmax /Km is 11fold as compared to reaction with coenzyme F420 | Methanobacterium formicicum | CO2 + FADH2 | - |
? | |
| formate + FMN | specific activity 9.1fold lower than for the reaction with coenzyme F420. Vmax /Km is 6.4fold as compared to reaction with coenzyme F420 | Methanobacterium formicicum | CO2 + FMNH2 | - |
? | |
| formate + oxidized coenzyme F420 | no activity with NAD+ or NADP* | Methanobacterium formicicum | CO2 + reduced coenzyme F420 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Methanobacterium formicicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assayed with coenzyme F420 | Methanobacterium formicicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8.3 | pH 7.3: about 40% of maximal activity, pH 8.3: about 70% of maximal activity | Methanobacterium formicicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
cyanide | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
| 0.039 | - |
azide | pH 7.5, temperature not specified in the publication | Methanobacterium formicicum | |
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