Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. SG-MS2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00117 | - |
(+)-pinoresinol | pH 7.2, 28°C | Pseudomonas sp. SG-MS2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. SG-MS2 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.2 | - |
pH 7.2, 28°C | Pseudomonas sp. SG-MS2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-pinoresinol + 2 oxidized azurin + H2O | - |
Pseudomonas sp. SG-MS2 | (+)-6-hydroxypinoresinol + 2 reduced azurin + 2 H+ | - |
? | |
(+)-pinoresinol + 2,6-dichlorophenolindophenol + phenazine methosulfate + H2O | - |
Pseudomonas sp. SG-MS2 | (+)-6-hydroxypinoresinol + reduced 2,6-dichlorophenolindophenol + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BLX42_10690 | gene name, flavoprotein component | Pseudomonas sp. SG-MS2 |
BLX42_10695 | gene name, cytochrome component | Pseudomonas sp. SG-MS2 |
pinAB | - |
Pseudomonas sp. SG-MS2 |
pinoresinol alpha-hydroxylase | - |
Pseudomonas sp. SG-MS2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | the flavoprotein component has a covalently attached flavin that it is attached to H411 through an alpha-N3-histidyl-FAD linkage | Pseudomonas sp. SG-MS2 |
Organism | Comment | Expression |
---|---|---|
Pseudomonas sp. SG-MS2 | the enzyme is overexpressed upon exposure to (+)-pinoresinol, along with 45 other proteins | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the pinoresinol-oxidizing enzyme in Pseudomonas sp. SG-MS2 is a flavocytochrome complex produced by the translationally coupled BLX42_10690 and BLX42_10695 genes | Pseudomonas sp. SG-MS2 |