Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.9.1 extracted from

  • Keat, M.J.; Hopper, D.J.
    p-Cresol and 3,5-xylenol methylhydroxylases in Pseudomonas putida N.C.I.B. 9896 (1978), Biochem. J., 175, 649-658.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.9.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 4-Cresol hydroxylase B Pseudomonas putida
0.015
-
 4-Cresol hydroxylase B, 4-hydroxybenzyl alcohol Pseudomonas putida
0.016
-
 4-Cresol hydroxylase A Pseudomonas putida
0.027
-
 4-Hydroxybenzyl alcohol hydroxylase A Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
58000
-
 2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
99000
-
 hydroxylase B, equilibrium sedimentation Pseudomonas putida
100000
-
 hydroxylase B, gel filtration Pseudomonas putida
108000
-
 hydroxylase A, gel filtration Pseudomonas putida
114000
-
 hydroxylase A, equilibrium sedimentation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida
-
 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B
-
Pseudomonas putida NCIB 9869
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida
6.78
-
 hydroxylase B Pseudomonas putida
9.95
-
 hydroxylase A Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-xylenol + acceptor + H2O i.e. 2,4-dimethylphenol Pseudomonas putida 3-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
3,4-dimethylphenol + acceptor + H2O
-
 Pseudomonas putida 2-methyl-4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O
-
 Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-cresol + acceptor + H2O
-
 Pseudomonas putida NCIB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-ethylphenol + acceptor + H2O
-
 Pseudomonas putida 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
 ?
4-ethylphenol + acceptor + H2O
-
 Pseudomonas putida NCIB 9869 1-(4'-hydroxyphenyl)ethanol + reduced acceptor
-
 ?
4-hydroxybenzyl alcohol + acceptor
-
 Pseudomonas putida 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
4-hydroxybenzyl alcohol + acceptor
-
 Pseudomonas putida NCIB 9869 4-hydroxybenzaldehyde + reduced acceptor
-
 ?
additional information no activity with 2,3-xylenol and 2,5-xylenol Pseudomonas putida ?
-
 ?
additional information no activity with 2-cresol Pseudomonas putida ?
-
 ?
additional information no activity with 2,3-xylenol and 2,5-xylenol Pseudomonas putida NCIB 9869 ?
-
 ?
additional information no activity with 2-cresol Pseudomonas putida NCIB 9869 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida
dimer 2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol Pseudomonas putida

Synonyms

Synonyms Comment Organism
p-cresol methylhydroxylase A 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B Pseudomonas putida
p-cresol methylhydroxylase B 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - formation of hydroxylase A - and on 4-cresol - formation of hydroxylase B Pseudomonas putida