KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
4-Cresol | hydroxylase B | Pseudomonas putida | |
0.015 | - |
4-Cresol | hydroxylase B, 4-hydroxybenzyl alcohol | Pseudomonas putida | |
0.016 | - |
4-Cresol | hydroxylase A | Pseudomonas putida | |
0.027 | - |
4-Hydroxybenzyl alcohol | hydroxylase A | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol | Pseudomonas putida |
58000 | - |
2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol | Pseudomonas putida |
99000 | - |
hydroxylase B, equilibrium sedimentation | Pseudomonas putida |
100000 | - |
hydroxylase B, gel filtration | Pseudomonas putida |
108000 | - |
hydroxylase A, gel filtration | Pseudomonas putida |
114000 | - |
hydroxylase A, equilibrium sedimentation | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | - |
2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B | - |
Pseudomonas putida NCIB 9869 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
6.78 | - |
hydroxylase B | Pseudomonas putida |
9.95 | - |
hydroxylase A | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-xylenol + acceptor + H2O | i.e. 2,4-dimethylphenol | Pseudomonas putida | 3-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
3,4-dimethylphenol + acceptor + H2O | - |
Pseudomonas putida | 2-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-cresol + acceptor + H2O | - |
Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-cresol + acceptor + H2O | - |
Pseudomonas putida NCIB 9869 | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-ethylphenol + acceptor + H2O | - |
Pseudomonas putida | 1-(4'-hydroxyphenyl)ethanol + reduced acceptor | - |
? | |
4-ethylphenol + acceptor + H2O | - |
Pseudomonas putida NCIB 9869 | 1-(4'-hydroxyphenyl)ethanol + reduced acceptor | - |
? | |
4-hydroxybenzyl alcohol + acceptor | - |
Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
4-hydroxybenzyl alcohol + acceptor | - |
Pseudomonas putida NCIB 9869 | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
additional information | no activity with 2,3-xylenol and 2,5-xylenol | Pseudomonas putida | ? | - |
? | |
additional information | no activity with 2-cresol | Pseudomonas putida | ? | - |
? | |
additional information | no activity with 2,3-xylenol and 2,5-xylenol | Pseudomonas putida NCIB 9869 | ? | - |
? | |
additional information | no activity with 2-cresol | Pseudomonas putida NCIB 9869 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, hydroxylase A, SDS-PAGE after incubation with mercaptoethanol | Pseudomonas putida |
dimer | 2 * 56000, hydroxylase B, SDS-PAGE after incubation with mercaptoethanol | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
p-cresol methylhydroxylase A | 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - hydroxylase A - and 4-cresol - hydroxylase B | Pseudomonas putida |
p-cresol methylhydroxylase B | 2 enzyme forms with various MW and Km are formed during growth on 3,5-xylenol - formation of hydroxylase A - and on 4-cresol - formation of hydroxylase B | Pseudomonas putida |