BRENDA - Reference to 1.17.9.1; Id = 674335

membrane

a tightly membrane-bound PCMH complex exists in anaerobic organisms in cotrast to aerobic ones, where the enzyme is soluble and periplasmic, overview

Geobacter metallireducens

16020

-

Fe2+

a cytochrome c-containing enzyme

Geobacter metallireducens

4-cresol + acceptor + H2O

Geobacter metallireducens

the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction

4-hydroxybenzyl alcohol + reduced acceptor

-

?

additional information

Geobacter metallireducens

pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview

?

-

Geobacter metallireducens

-

two isozymes encoded by genes pcmI and pcmJ

-

0.01

-

cell extract, substrate 4-hydroxybenzyl alcohol

Geobacter metallireducens

0.025

-

cell extract, substrate 4-cresol

Geobacter metallireducens

4-cresol + acceptor + H2O

the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + reduced acceptor

-

?

4-cresol + ferricenium + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + ferrocenium

-

?

4-cresol + NAD(P)+ + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + NAD(P)H

-

?

4-cresol + phenazine methosulfate + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + reduced phenazine methosulfate

-

?

additional information

pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview

674335

Geobacter metallireducens

?

-

additional information

an unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of 4-hydroxybenzyl alcohol oxidation, overview, no activity with 4-hydroxybenzoate, benzoate, and acetate

674335

Geobacter metallireducens

?

-

More

unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of p-hydroxybenzyl alcohol oxidation, overview

Geobacter metallireducens

p-cresol methylhydroxylase

-

Geobacter metallireducens

30

-

assay at

Geobacter metallireducens

7.6

-

assay at

Geobacter metallireducens

cytochrome c

active in 4-cresol oxidation

Geobacter metallireducens

FAD

-

Geobacter metallireducens

menaquinone

active in 4-hydroxybenzyl alcohol oxidation

Geobacter metallireducens

NAD+

-

Geobacter metallireducens

NADP+

-

Geobacter metallireducens

phenazine methosulfate

-

Geobacter metallireducens

cytochrome c

active in 4-cresol oxidation

Geobacter metallireducens

FAD

-

Geobacter metallireducens

menaquinone

active in 4-hydroxybenzyl alcohol oxidation

Geobacter metallireducens

NAD+

-

Geobacter metallireducens

NADP+

-

Geobacter metallireducens

phenazine methosulfate

-

Geobacter metallireducens

membrane

a tightly membrane-bound PCMH complex exists in anaerobic organisms in cotrast to aerobic ones, where the enzyme is soluble and periplasmic, overview

Geobacter metallireducens

16020

-

Fe2+

a cytochrome c-containing enzyme

Geobacter metallireducens

4-cresol + acceptor + H2O

Geobacter metallireducens

the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction

4-hydroxybenzyl alcohol + reduced acceptor

-

?

additional information

Geobacter metallireducens

pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview

?

-

0.01

-

cell extract, substrate 4-hydroxybenzyl alcohol

Geobacter metallireducens

0.025

-

cell extract, substrate 4-cresol

Geobacter metallireducens

4-cresol + acceptor + H2O

the obligately anaerobic Geobacter metallireducens uses the 4-cresol degradation pathway of denitrifying bacteria, PCMH catalyzes both 4-cresol hydroxylation and 4-hydroxybenzyl alcohol oxidation to the corresponding aldehyde, unusually it is located in the membrane fraction

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + reduced acceptor

-

?

4-cresol + ferricenium + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + ferrocenium

-

?

4-cresol + NAD(P)+ + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + NAD(P)H

-

?

4-cresol + phenazine methosulfate + H2O

PCMH catalyzes both p-cresol hydroxylation and p-hydroxybenzyl alcohol oxidation and uses NAD+, NADP+, ferricenium, or phenazine methosulfate as electron acceptors

674335

Geobacter metallireducens

4-hydroxybenzyl alcohol + reduced phenazine methosulfate

-

?

additional information

pathways of 4-cresol metabolism to benzoyl-CoA in anaerobic bacteria, detailed overview

674335

Geobacter metallireducens

?

-

additional information

an unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of 4-hydroxybenzyl alcohol oxidation, overview, no activity with 4-hydroxybenzoate, benzoate, and acetate

674335

Geobacter metallireducens

?

-

More

unusual asymmetric architecture, with an unique alphaalpha'beta2 subunit composition, and the membrane association of PCMH might be important for alternative electron transfer routes to either cytochrome c, in the case of 4-cresol oxidation, or to menaquinone, in the case of p-hydroxybenzyl alcohol oxidation, overview

Geobacter metallireducens

30

-

assay at

Geobacter metallireducens

7.6

-

assay at

Geobacter metallireducens

744935

Chaurasia

Genetic evidence that the deg ...

Geobacter metallireducens

FEMS Microbiol. Lett.

362

fnv145

2015

-

1

-

1

-

4

-

1

-

3

-

1

-

1

-

1

-

726788

Chen

-

The catabolism of 2,4-xylenol ...

Pseudomonas putida

Appl. Microbiol. Biotechnol.

98

1349-1356

2014

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

-

2

-

1

-

1

-

744141

Chen

The catabolism of 2,4-xylenol ...

Pseudomonas putida, Pseudomonas putida NCIMB 9866

Appl. Microbiol. Biotechnol.

98

1349-1356

2014

-

1

-

4

-

3

-

1

-

8

-

4

-

1

-

4

-

1

-

8

-

1

-

715044

Joesaar

Diversity of the transcription ...

Pseudomonas fluorescens, Pseudomonas fluorescens PC24

FEMS Microbiol. Ecol.

72

464-475

2010

-

1

-

4

-

7

-

4

-

4

-

1

-

2

-

4

-

4

-

2

-

2

4

3

-

687426

Johannes

Purification and characterizat ...

Geobacter metallireducens

J. Bacteriol.

190

6493-6500

2008

-

1

2

1

-

2

-

4

-

1

-

1

-

5

1

2

-

2

-

2

-

1

2

1

-

2

-

1

-

1

-

5

1

-

674335

Peters

Genes, enzymes, and regulation ...

Geobacter metallireducens

J. Bacteriol.

189

4729-4738

2007

-

1

-

2

-

5

-

2

-

6

1

-

1

-

6

-

6

-

1

-

2

-

2

-

6

1

-

1

-

658110

Cunane

p-Cresol methylhydroxylase: al ...

Pseudomonas putida

Biochemistry

44

2963-2973

2005

-

1

-

3

1

-

1

-

1

-

2

1

-

1

-

1

-

3

1

-

1

-

2

1

-

658990

Efimov

Relationship between charge-tr ...

Pseudomonas putida

J. Am. Chem. Soc.

127

732-741

2005

-

1

-

2

1

-

1

-

1

-

1

-

2

-

3

-

1

3

-

2

1

-

1

-

1

-

2

-

658018

Efimov

A study of the spectral and re ...

Pseudomonas putida

Biochemistry

43

10532-10546

2004

-

1

-

1

-

2

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

2

1

-

1

-

1

-

2

-

658077

Efimov

Insight into covalent flavinyl ...

Pseudomonas putida, Pseudomonas putida NCIMB 9869

Biochemistry

43

6138-6148

2004

-

1

-

1

-

2

-

3

-

1

-

2

1

-

2

-

1

-

1

-

1

-

2

-

1

-

2

1

-

2

-

437885

Efimov

Effects of noncovalent and cov ...

Pseudomonas putida

Biochemistry

40

2155-2166

2001

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

437882

Cunane

Structures of the flavocytochr ...

Pseudomonas putida

J. Mol. Biol.

295

357-374

2000

-

1

-

1

-

4

-

3

-

2

-

2

1

-

1

-

3

-

437886

Cronin

Heterologous expression in Pse ...

Pseudomonas putida

Protein Expr. Purif.

19

74-83

2000

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

1

-

437883

Engst

Properties of p-cresol methylh ...

Pseudomonas putida

Biochemistry

38

16620-16628

1999

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

437884

Kim

The cytochrome subunit is nece ...

Pseudomonas putida

J. Biol. Chem.

270

31202-31209

1995

-

1

-

4

-

1

-

1

-

2

-

2

-

1

-

1

-

1

-

437876

Kim

Cloning, sequencing, and expre ...

Pseudomonas putida, Pseudomonas putida NCIB 9866, Pseudomonas putida NCIB 9869

J. Bacteriol.

176

6349-6361

1994

-

1

-

14

-

1

-

3

-

2

-

1

2

-

1

-

3

-

288313

Bossert

Anaerobic oxidation of p-creso ...

denitrifying bacterium, denitrifying bacterium PC-07

J. Bacteriol.

171

2956-2962

1989

-

6

-

1

-

4

-

1

-

2

-

14

-

2

-

2

-

6

-

1

-

1

-

2

-

14

-

437879

McIntire

Steady-state and stopped-flow ...

Pseudomonas putida, Pseudomonas putida NCIB 9869

Biochemistry

26

4107-4117

1987

-

7

-

1

-

4

-

1

-

4

-

1

-

437877

Shamala

Structure of an intermolecular ...

Pseudomonas putida

Proc. Natl. Acad. Sci. USA

83

4626-4630

1986

-

2

-

1

-

1

-

2

-

2

-

2

-

1

-

437878

McIntire

Amino acid and sequence analys ...

Pseudomonas putida

Biochemistry

25

5975-5981

1986

-

2

-

2

-

1

-

2

-

1

-

437871

Hopper

Periplasmic location of p-cres ...

Pseudomonas putida

FEBS Lett.

182

485-488

1985

-

2

-

1

-

3

-

2

-

2

-

1

-

2

-

437872

Bhattacharyya

Laser-flash-photolysis studies ...

Pseudomonas putida

Biochem. J.

228

337-345

1985

-

2

-

1

-

2

-

2

-

1

-

437873

Shamala

Preliminary X-ray study of p-c ...

Pseudomonas putida

J. Mol. Biol.

183

517-518

1985

-

1

-

2

-

1

-

1

-

1

-

437874

McIntire

p-Cresol methylhydroxylase. as ...

Pseudomonas putida

Biochem. J.

228

325-335

1985

-

7

-

3

-

21

-

1

-

1

-

1

-

7

-

21

-

1

-

437875

Koerber

Resolution of the flavocytochr ...

Pseudomonas putida

Biochemistry

24

5276-5280

1985

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

437869

Causer

-

Azurin from Pseudomonas putida ...

Pseudomonas putida, Pseudomonas putida NCIB 9869

Biochem. Soc. Trans.

12

1131-1132

1984

-

1

2

-

7

-

4

-

1

2

-

4

-

437870

McIntire

Stereochemistry of 1-(4-hydrox ...

Pseudomonas putida

Biochem. J.

224

617-621

1984

-

2

-

2

-

2

-

437868

Hopper

Redox potential of the cytochr ...

Comamonas testosteroni, Comamonas testosteroni NCIB 8955, Pseudomonas alcaligenes, Pseudomonas alcaligenes NCIB 9867, Pseudomonas putida, Pseudomonas putida NCIB 9866, Pseudomonas putida NCIB 9869

FEBS Lett.

161

100-102

1983

-

17

-

7

-

3

-

3

-

7

-

437867

McIntire

8 alpha-(O-Tyrosyl)flavin aden ...

Pseudomonas putida

Biochemistry

20

3068-3075

1981

-

2

-

1

-

1

-

1

-

1

-

437866

McIntire

8 alpha-O-Tyrosyl-FAD: a new f ...

Pseudomonas putida

J. Biol. Chem.

255

6553-6555

1980

-

1

-

1

-

3

-

3

-

1

-

437865

Hopper

Incorporation of [18O]water in ...

Pseudomonas putida

Biochem. J.

175

345-347

1978

-

2

-

3

-

3

-

437880

Keat

p-Cresol and 3,5-xylenol methy ...

Pseudomonas putida, Pseudomonas putida NCIB 9869

Biochem. J.

175

649-658

1978

-

4

-

6

-

9

-

1

-

3

-

12

1

2

-

4

-

6

-

1

-

3

-

12

1

-

437864

Hopper

The purification and propertie ...

Pseudomonas putida

Biochem. J.

167

155-162

1977

-

5

-

2

1

-

3

-

1

-

1

8

1

-

2

-

2

-

5

-

2

1

-

1

-

1

8

1

-

Genes, enzymes, and regulation of para-cresol metabolism in Geobacter metallireducens

Data extracted from this reference: