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Literature summary for 1.17.7.3 extracted from

  • Volke, D.C.; Rohwer, J.; Fischer, R.; Jennewein, S.
    Investigation of the methylerythritol 4-phosphate pathway for microbial terpenoid production through metabolic control analysis (2019), Microb. Cell Fact., 18, 192.
    View publication on PubMed
Search for more literature for 1.17.7.3

Protein Variants

Protein Variants Comment Organism
additional information simple overexpression of IspG and IspH (EC 1.17.7.4) does not increase the flux towards terpenoids via the MEP pathway Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced flavodoxin Escherichia coli
-
 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized flavodoxin
-
 ?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced flavodoxin Escherichia coli K12
-
 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized flavodoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P62620
-
-
Escherichia coli K12 P62620
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced flavodoxin
-
 Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized flavodoxin
-
 ?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced flavodoxin
-
 Escherichia coli K12 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized flavodoxin
-
 ?
additional information turnover numbers of IspG are measured using artificial reducing agents Escherichia coli ?
-
 ?
additional information turnover numbers of IspG are measured using artificial reducing agents Escherichia coli K12 ?
-
 ?

Synonyms

Synonyms Comment Organism
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
-
 Escherichia coli
HMBPP synthase
-
 Escherichia coli
IspG
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
flavodoxin
-
 Escherichia coli
additional information IspG needs several cofactors for its activity Escherichia coli

General Information

General Information Comment Organism
metabolism terpenoids are of high interest as chemical building blocks and pharmaceuticals. In microbes, terpenoids can be synthesized via the methylerythritol phosphate (MEP) or mevalonate (MVA) pathways. In the MEP pathway of Escherichia coli recombinantly expressing isoprene synthase (ispS) from Populus alba, the endogenous expression of 1-deoxyxylulose 5-phosphate synthase (Dxs) controls the flux through the MEP pathway increasing the 2-C-methyl-d-erythritol-2,4-cyclopyrophosphate (MEcPP) concentration to a higher amount compared to the other MEP pathway intermediates. Thus, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (IspG), which consumes MEcPP, becomes saturated and therefore limits the flux towards isoprene. The higher intracellular concentrations of MEcPP lead to the efflux of this intermediate into the growth medium. Proteomic and metabolomic analysis of the MEP pathway, overview Escherichia coli
additional information simple overexpression of IspG and IspH (EC 1.17.7.4) does not increase the flux towards terpenoids via the MEP pathway Escherichia coli