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Literature summary for 1.17.7.3 extracted from

  • Seemann, M.; Tse Sum Bui, B.; Wolff, M.; Tritsch, D.; Campos, N.; Boronat, A.; Marquet, A.; Rohmer, M.
    Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein (2002), Angew. Chem. Int. Ed. Engl., 41, 4337-4339.
    View publication on PubMed
Search for more literature for 1.17.7.3

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S]2+ cluster the purified protein is inactive. It is activated after reconstitution of its Fe/S cluster Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin Escherichia coli the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P62620
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged enzyme. The purified protein is inactive. It is activated after reconstitution of its Fe/S cluster Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
-
 Escherichia coli (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
GcpE ambiguous Escherichia coli

General Information

General Information Comment Organism
metabolism the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis Escherichia coli