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Literature summary for 1.17.7.1 extracted from

  • Li, D.; Wang, Q.; Chen, S.; Liu, H.; Pan, K.; Li, J.; Luo, C.; Wang, H.
    De novo assembly and analysis of Polygonatum cyrtonema Hua and identification of genes involved in polysaccharide and saponin biosynthesis (2022), BMC Genomics, 23, 195.
    View publication on PubMed
Search for more literature for 1.17.7.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ispG, quantitative real-time PCR enzyme expression analysis, transcriptome analysis Polygonatum cyrtonema

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Polygonatum cyrtonema

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin Polygonatum cyrtonema
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 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Polygonatum cyrtonema
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
-
 Polygonatum cyrtonema (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
-
 ?

Synonyms

Synonyms Comment Organism
(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
-
 Polygonatum cyrtonema
IspG
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 Polygonatum cyrtonema

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Polygonatum cyrtonema

General Information

General Information Comment Organism
metabolism the content of total polysaccharide and total saponin is positively correlated with the expression patterns of several enzymes including the 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF, EC 4.6.1.12), (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (ispG), and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH, EC 1.17.7.4), transcriptome analysis and determination of a link between polysaccharides and saponin biosynthesis and gene expression, overview. For saponin biosynthesis pathway, the genes encoding HMGS, MVK, ispF, ispG, ispH and FPPS enzymes are high expressed in three-year rhizomes, and these gene expression pattern is consistent with the accumulation pattern of total saponin with the rhizome development. It seems that MEP and MVA pathway all participate in the saponin biosynthesis Polygonatum cyrtonema