Literature summary for 1.17.5.3 extracted from

Enoch, H.G.; Lester, R.L.
The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli (1975), J. Biol. Chem., 250, 6693-7705.

Inhibitors

Inhibitors	Comment	Organism
azide	-	Escherichia coli
cyanide	-	Escherichia coli
iodoacetamide	-	Escherichia coli
O2	-	Escherichia coli
p-hydroxymercuribenzoate	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	formate	30°C, per mol of heme	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	bound to	Escherichia coli	16020	-

Metals/Ions

Metals/Ions	Comment	Organism
Fe	the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide	Escherichia coli
Mo	the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide	Escherichia coli
additional information	contains neither FAD nor FMN	Escherichia coli
Se	the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide. The alpha polypeptide contains significant amounts of selenium	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE	Escherichia coli
32000	-	x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE	Escherichia coli
110000	-	x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE	Escherichia coli
590000	-	gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Oxidation Stability

Oxidation Stability	Organism
formate dehydrogenase activity is lost when the enzyme is exposed to oxygen. This instability is enhanced in low ionic strength buffers, detergents, at temperatures above 0°C, and at pH greater than 7	Escherichia coli
formate dehydrogenase is extremely sensitive to inactivation by oxygen in the presence of formate. Purified formate dehydrogenase is completely inactivated by aerobic incubation in 75 mM sodium phosphate, pH 7, for 1 h at 20°C in the presence of 50 mM formate, while only 40% of the activity is lost when the same incubation is carried out in the absence of formate. No activity is lost when the same incubation is carried out anaerobically even in the presence of formate	Escherichia coli

Organism

formate dehydrogenase activity is lost when the enzyme is exposed to oxygen. This instability is enhanced in low ionic strength buffers, detergents, at temperatures above 0°C, and at pH greater than 7

Escherichia coli

formate dehydrogenase is extremely sensitive to inactivation by oxygen in the presence of formate. Purified formate dehydrogenase is completely inactivated by aerobic incubation in 75 mM sodium phosphate, pH 7, for 1 h at 20°C in the presence of 50 mM formate, while only 40% of the activity is lost when the same incubation is carried out in the absence of formate. No activity is lost when the same incubation is carried out anaerobically even in the presence of formate

Escherichia coli

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
196	-	-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
formate + 2,3-dimethoxy-5-methyl-6-(farnesylfarnesyl)-1,4-benzoquinone	-	Escherichia coli	CO2 + ?	-	?
formate + 2,6-dichlorophenolindophenol	dichlorophenylindophenol reduction in presence of phenazine methosulfate	Escherichia coli	CO2 + reduced 2,6-dichlorophenolindophenol	-	ir
formate + benzyl viologen	-	Escherichia coli	CO2 + reduced benzyl viologen	-	?
formate + ferricyanide	-	Escherichia coli	CO2 + ferrocyanide + H+	-	?
formate + methyl viologen	-	Escherichia coli	CO2 + reduced methyl viologen	-	ir
formate + methylene blue	-	Escherichia coli	CO2 + reduced methylene blue	-	?
formate + nitroblue tetrazolium	-	Escherichia coli	CO2 + reduced nitroblue tetrazolium	-	?

Subunits

Subunits	Comment	Organism
?	x * 110000 (alpha) + x * 32000 (beta) + x * 20000 (gamma), the molar ratio of alpha:beta:gamma is 1:1.2:0.55, SDS-PAGE	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	pH 8.5, 0.75 M Tris-HCl, anaerobic condition, stable for 15 min	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
563	-	formate	30°C, per mol of heme	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
heme	the enzyme contains (in relative molar amounts): 1.0 heme, 0.95 molybdenum, 0.96 selenium, 14 non-heme iron, and 13 acid-labile sulfide	Escherichia coli