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Literature summary for 1.17.5.1 extracted from
- Phenylacetyl-CoA:acceptor oxidoreductase, a membrane-bound molybdenum-iron-sulfur enzyme involved in anaerobic metabolism of phenylalanine in the denitrifying bacterium Thauera aromatica (1999), Eur. J. Biochem., 262, 507-515.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CN- | reversible inactivation by a low concentration of cyanide | Thauera aromatica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
phenylacetyl-CoA | pH 7.5, 30°C, 2,6-dichlorophenolindophenol as electron acceptor | Thauera aromatica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Thauera aromatica | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
alpha2beta2gamma2, 2 * 93000 + 2 * 27000 + 2 * 26000, SDS-PAGE | Thauera aromatica |
| 27000 | - |
alpha2beta2gamma2, 2 * 93000 + 2 * 27000 + 2 * 26000, SDS-PAGE | Thauera aromatica |
| 93000 | - |
alpha2beta2gamma2, 2 * 93000 + 2 * 27000 + 2 * 26000, SDS-PAGE | Thauera aromatica |
| 280000 | - |
gel filtration | Thauera aromatica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenylacetyl-CoA + H2O + ubiquinone | Thauera aromatica | ubiquinone is most likely the natural electron acceptor, involved in anaerobic metabolism of phenylalanine, catalyzes the first step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the second step being carried out by EC 3.1.2.25 | phenylglyoxylyl-CoA + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera aromatica | - |
- |
- |
| Thauera aromatica | - |
strain DSM 6984 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| remarkably stable with respect to oxygen | Thauera aromatica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 12fold, partial | Thauera aromatica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
pH 7.5, 30°C, 2,6-dichlorophenolindophenol as electron acceptor | Thauera aromatica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% (w/v) glycerol, 0.1% (w/v) lauryldimethylamine oxide, partially purified enzyme, several weeks, stable | Thauera aromatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrates: phenylacetate, acetyl-CoA, benzoyl-CoA, propionyl-CoA, crotonyl-CoA, succinyl-CoA, 3-hydroxybenzoyl-CoA | Thauera aromatica | ? | - |
? | |
| phenylacetyl-CoA + H2O + 2,6-dichlorophenolindophenol | uses 2,6-dichlorophenolindophenol as artificial electron acceptor | Thauera aromatica | phenylglyoxylyl-CoA + reduced 2,6-dichlorophenolindophenol | - |
? | |
| phenylacetyl-CoA + H2O + duroquinone | at 0.3 mM duroquinone, 150% of the activity with 0.25 mM 2,6-dichlorophenolindophenol | Thauera aromatica | phenylglyoxylyl-CoA + duroquinol | - |
? | |
| phenylacetyl-CoA + H2O + menadione | at 0.3 mM menadione, 60% of the activity with 0.25 mM 2,6-dichlorophenolindophenol | Thauera aromatica | phenylglyoxylyl-CoA + menadiol | - |
? | |
| phenylacetyl-CoA + H2O + quinone | - |
Thauera aromatica | phenylglyoxylyl-CoA + quinol | - |
? | |
| phenylacetyl-CoA + H2O + quinone | four-electron oxidation, catalyzes an alpha-oxidation without utilizing molecular oxygen, ubiquinone is most likely the natural electron acceptor, specific for phenylacetyl-CoA, enzyme preparation catalyzes the reaction phenylacetyl-CoA + 2 H2O + 2 quinone = phenylglyoxylate + 2 quinone H2 + CoASH with phenylglyoxylyl-CoA as free intermediate, which is subsequently hydrolyzed | Thauera aromatica | phenylglyoxylyl-CoA + quinol | - |
? | |
| phenylacetyl-CoA + H2O + ubiquinone | ubiquinone is most likely the natural electron acceptor, involved in anaerobic metabolism of phenylalanine, catalyzes the first step in the conversion of phenylacetyl-CoA to phenylglyoxylate, the second step being carried out by EC 3.1.2.25 | Thauera aromatica | phenylglyoxylyl-CoA + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | alpha2beta2gamma2, 2 * 93000 + 2 * 27000 + 2 * 26000, SDS-PAGE | Thauera aromatica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Thauera aromatica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
phenylacetyl-CoA | pH 7.5, 30°C, duroquinone as electron acceptor | Thauera aromatica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
2,6-dichlorophenolindophenol as electron acceptor | Thauera aromatica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| iron-sulfur centre | molybdenum-iron-sulfur enzyme, contains 30 mol Fe and 25 mol acid-labile sulfur per mol of native enzyme | Thauera aromatica | |
| molybdenum cofactor | molybdenum-iron-sulfur enzyme, contains 0.66 mol Mo per mol of native enzyme | Thauera aromatica | |
| molybdopterin | presence of a molybdopterin cofactor, contains 0.66 mol Mo per mol of native enzyme | Thauera aromatica | |
| additional information | catalyzes an alpha-oxidation without utilizing molecular oxygen | Thauera aromatica |
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