Cloned (Comment) | Organism |
---|---|
gene VKORC1 Y139F, DNA and amino acid sequence determination, genotyping, semiquantitative expression analysis | Rattus norvegicus |
gene VKORC1, DNA and amino acid sequence determination, genotyping, semiquantitative expression analysis | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y139F | the natural occuring mutation confers resistance to enzyme inhibitor warfarin, the mutant rats do not show vitamin K deficiency | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
warfarin | the mutant Y139F strain is resistant to warfarin; wild-type rats | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.0195 | - |
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone | pH 7.4, 25°C | Rattus norvegicus | |
0.0577 | - |
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone | pH 7.4, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | Rattus norvegicus | i.e. vitamin K 2,3-epoxide | 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol | i.e. vitamin K | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
gene VKORC1, mutant Y139F | - |
Rattus norvegicus | Q6TEK4 | warfarin-susceptible rats; gene VKORC1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | i.e. vitamin K 2,3-epoxide | Rattus norvegicus | 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol | i.e. vitamin K | ? |
Synonyms | Comment | Organism |
---|---|---|
VKOR | - |
Rattus norvegicus |
VKORC1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
0.00072 | - |
warfarin | pH 7.4, 25°C | Rattus norvegicus | |
0.029 | - |
warfarin | pH 7.4, 25°C | Rattus norvegicus |