BRENDA - Enzyme Database show
show all sequences of 1.17.4.2

Inactivation of Lactobacillus leichmannii ribonucleotide reductase by 2',2'-difluoro-2'-deoxycytidine 5'-triphosphate: covalent modification

Lohman, G.J.; Stubbe, J.; Biochemistry 49, 1404-1417 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C119S
site-directed mutagenesis
Lactobacillus leichmannii
Inhibitors
Inhibitors
Commentary
Organism
Structure
2',2'-difluoro-2'-deoxycytidine 5'-triphosphate
F2CTP, a clinically used anti-cancer drug, synthesis, overview. Causes rapid enzyme inactivation through covalent modification, mechanism of inactivation, analysis by mass spectrometry and radio- or deuterium-labeled compounds, overview. C119 is involved in the reaction
Lactobacillus leichmannii
2'-chloro-2'-deoxy-UTP
-
Lactobacillus leichmannii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
-
Lactobacillus leichmannii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactobacillus leichmannii
RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides using adenosylcobalamin as a cofactor
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus leichmannii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + adenosylcobalamin
-
714186
Lactobacillus leichmannii
?
-
-
-
?
additional information
RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides using adenosylcobalamin as a cofactor
714186
Lactobacillus leichmannii
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 76000
Lactobacillus leichmannii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Lactobacillus leichmannii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactobacillus leichmannii
Cofactor
Cofactor
Commentary
Organism
Structure
adenosylcobalamin
-
Lactobacillus leichmannii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
adenosylcobalamin
-
Lactobacillus leichmannii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C119S
site-directed mutagenesis
Lactobacillus leichmannii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2',2'-difluoro-2'-deoxycytidine 5'-triphosphate
F2CTP, a clinically used anti-cancer drug, synthesis, overview. Causes rapid enzyme inactivation through covalent modification, mechanism of inactivation, analysis by mass spectrometry and radio- or deuterium-labeled compounds, overview. C119 is involved in the reaction
Lactobacillus leichmannii
2'-chloro-2'-deoxy-UTP
-
Lactobacillus leichmannii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
-
Lactobacillus leichmannii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactobacillus leichmannii
RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides using adenosylcobalamin as a cofactor
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CTP + adenosylcobalamin
-
714186
Lactobacillus leichmannii
?
-
-
-
?
additional information
RTPR catalyzes the conversion of nucleoside triphosphates to deoxynucleotides using adenosylcobalamin as a cofactor
714186
Lactobacillus leichmannii
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 76000
Lactobacillus leichmannii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Lactobacillus leichmannii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactobacillus leichmannii
Other publictions for EC 1.17.4.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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746494
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715583
Pontarin
Deoxyribonucleotide metabolism ...
Homo sapiens
J. Biol. Chem.
286
11132-11140
2011
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2
2
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716317
Logan
Closing the circle on ribonucl ...
Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhimurium
Nat. Struct. Mol. Biol.
18
251-253
2011
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3
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3
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6
4
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3
3
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716563
Halimi
COP9 signalosome subunit 7 fro ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Mol. Biol.
77
77-89
2011
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1
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1
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1
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3
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4
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6
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1
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4
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1
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4
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1
1
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728091
Manzerova
Investigating the intermediate ...
Lactobacillus leichmannii
J. Magn. Reson.
213
32-45
2011
-
1
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3
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714185
Lohman
Inactivation of Lactobacillus ...
Lactobacillus leichmannii
Biochemistry
49
1396-1403
2010
-
-
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1
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1
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1
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2
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2
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1
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1
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1
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1
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2
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1
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1
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714186
Lohman
Inactivation of Lactobacillus ...
Lactobacillus leichmannii
Biochemistry
49
1404-1417
2010
-
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1
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2
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1
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1
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2
1
1
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1
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715085
Holmgren
The use of thiols by ribonucle ...
Escherichia coli, Escherichia virus T4, Mus musculus
Free Radic. Biol. Med.
49
1617-1628
2010
-
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12
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4
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6
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3
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8
7
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11
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11
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12
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8
7
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4
4
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716377
Ylikallio
Ribonucleotide reductase is no ...
Mus musculus
Nucleic Acids Res.
38
8208-8218
2010
-
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1
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1
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1
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4
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1
3
3
1
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716674
Panosa
Ribonucleotide reductases of S ...
Salmonella enterica subsp. enterica serovar Typhimurium
PLoS ONE
5
e11328
2010
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2
2
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687196
Roca
Fumarate and nitrate reduction ...
Escherichia coli K-12
Int. Microbiol.
11
49-56
2008
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1
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687199
Odenike
Phase I study of the ribonucle ...
Homo sapiens
Invest. New Drugs
26
233-239
2008
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1
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688754
Karp
A phase I study of the novel r ...
Homo sapiens
Leuk. Res.
32
71-77
2008
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2
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689295
Saiko
Novel resveratrol analogs indu ...
Homo sapiens
Oncol. Rep.
19
1621-1626
2008
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671925
Lin
Excess ribonucleotide reductas ...
Homo sapiens
Biochem. Pharmacol.
73
760-772
2007
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672890
Madlener
Gallic acid inhibits ribonucle ...
Homo sapiens
Cancer Lett.
245
156-162
2007
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2
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673802
Camier
Visualization of ribonucleotid ...
Saccharomyces cerevisiae
Free Radic. Biol. Med.
42
1008-1016
2007
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1
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676528
Garton
crinkled leaves 8 - A mutation ...
Arabidopsis thaliana
Plant J.
50
118-127
2007
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1
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685023
Sigmond
The synergistic interaction of ...
Homo sapiens
Biochem. Pharmacol.
73
1548-1557
2007
-
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3
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1
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5
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5
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1
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686091
Cerqueira
Understanding ribonucleotide r ...
Escherichia coli
Chemistry
13
8507-8515
2007
-
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1
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1
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687180
Saiko
N-hydroxy-N-(3,4,5-trimethoxyp ...
Homo sapiens
Int. J. Oncol.
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688750
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Phase I and pharmacokinetic st ...
Homo sapiens
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673834
Pham
Regulation of the ribonucleoti ...
Aedes aegypti
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Regulation of mammalian ribonu ...
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676426
Wang
Arabidopsis ribonucleotide red ...
Arabidopsis thaliana
Plant Cell
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2006
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1
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659464
Torrents
Two proteins mediate class II ...
Pseudomonas aeruginosa
J. Biol. Chem.
280
16571-16578
2005
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1
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671912
Liu
The ribonucleotide reductase s ...
Homo sapiens
Biochem. Pharmacol.
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1288-1297
2005
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1
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659994
Larsson
Structural mechanism of allost ...
Thermotoga maritima
Nat. Struct. Mol. Biol.
11
1142-1149
2004
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1
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2
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-
657991
Chen
Epimerization at carbon-5' of ...
Lactobacillus leichmannii
Biochemistry
42
4578-4584
2003
1
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2
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2
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1
-
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-
660352
Logan
A metal-binding site in the ca ...
Escherichia coli, Escherichia coli JM109
Proc. Natl. Acad. Sci. USA
100
3826-3831
2003
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1
4
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33
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1
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2
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4
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1
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2
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-
-
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-
437906
Hoegbom
Crystal structure of the di-ir ...
Corynebacterium ammoniagenes
Biochemistry
41
1381-1389
2002
-
-
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1
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4
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6
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1
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6
-
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-
437904
Chabes
Yeast ribonucleotide reductase ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
97
2474-2479
2000
-
-
-
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1
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3
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1
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1
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1
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2
-
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-
-
-
-
-
-
-
-
437899
Jordan
Ribonucleotide reduction in Ps ...
Brevundimonas diminuta, Brevundimonas vesicularis, Burkholderia cepacia, Deinococcus radiodurans, Delftia acidovorans, Escherichia coli, Hydrogenophaga flava, Lactobacillus leichmannii, Mycobacterium tuberculosis, Paracoccus denitrificans, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas sp., Pseudomonas stutzeri, Ralstonia pickettii, Stenotrophomonas maltophilia, Xanthomonas campestris
J. Bacteriol.
181
3974-3980
1999
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17
-
17
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34
-
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4
-
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4
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17
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34
-
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-
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-
-
-
-
-
-
437900
Licht
Studies on the catalysis of ca ...
Escherichia coli, Lactobacillus leichmannii
Biochemistry
38
1221-1233
1999
-
-
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2
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2
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3
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5
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1
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1
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2
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2
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5
-
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-
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-
-
-
-
-
-
437901
Licht
Thermodynamic and kinetic stud ...
Lactobacillus leichmannii
Biochemistry
38
1234-1242
1999
-
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1
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2
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2
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1
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437902
Suto
-
Ribonucleoside triphosphate re ...
Lactobacillus leichmannii
Bioorg. Chem.
27
451-462
1999
1
-
1
-
-
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8
-
-
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1
-
1
-
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1
-
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2
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1
8
-
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1
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1
1
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8
8
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1
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1
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437903
Suto
Adenosylcobalamin-dependent ri ...
Lactobacillus leichmannii
Prep. Biochem. Biotechnol.
29
273-309
1999
1
-
1
1
-
-
-
-
-
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2
1
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2
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1
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4
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6
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1
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1
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1
1
1
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2
1
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1
-
-
-
-
4
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
437897
Jordan
B12-dependent ribonucleotide r ...
Archaeoglobus fulgidus, Chloroflexus aurantiacus, Deinococcus radiodurans, Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539, Escherichia coli, Lactobacillus leichmannii, Methanocaldococcus jannaschii, Mycobacterium tuberculosis, Pyrococcus furiosus, Thermoplasma acidophilum, Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
Proc. Natl. Acad. Sci. USA
94
13487-13492
1997
5
-
2
-
-
-
5
-
-
5
3
12
-
48
-
-
2
-
-
-
-
-
36
-
1
-
-
-
-
-
-
4
-
-
-
5
-
2
4
-
-
-
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5
-
-
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5
3
12
-
-
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2
-
-
-
-
36
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
437898
Tauer
The B12-dependent ribonucleoti ...
Anabaena sp., Escherichia coli, Halobacterium salinarum, Haloferax volcanii, Homo sapiens, Lactobacillus leichmannii, Mycobacterium tuberculosis, Thermoplasma acidophilum, Thermus aquaticus, Thermus aquaticus X1
Proc. Natl. Acad. Sci. USA
94
53-58
1997
2
-
1
-
-
-
-
-
-
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1
10
-
19
-
-
1
-
-
-
1
-
20
-
1
-
-
-
-
-
-
3
-
-
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2
-
1
3
-
-
-
-
-
-
-
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1
10
-
-
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1
-
-
1
-
20
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
437896
Booker
Coenzyme B12-dependent ribonuc ...
Lactobacillus leichmannii
Biochemistry
33
12676-12685
1994
-
-
1
-
-
-
-
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-
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1
1
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3
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-
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2
1
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-
-
-
-
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1
-
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-
1
1
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-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437895
Halicky
Immobilization of ribonucleoti ...
Kitasatospora aureofaciens
Collect. Czech. Chem. Commun.
54
2528-2541
1989
2
-
-
-
-
1
-
8
-
2
-
1
-
2
-
-
-
-
-
2
-
-
8
1
1
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
1
-
-
-
8
-
2
-
1
-
-
-
-
-
2
-
-
8
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
437894
Ashley
The mechanism of Lactobacillus ...
Lactobacillus leichmannii
J. Biol. Chem.
261
3958-3964
1986
-
-
-
-
-
-
-
-
-
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1
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2
-
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3
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1
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1
-
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-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437893
Gleason
Adenosylcobalamin-dependent ri ...
Anabaena sp., Anabaena sp. 7119, Escherichia coli, Lactobacillus leichmannii
J. Biol. Chem.
255
7728-7733
1980
6
-
-
-
-
-
2
1
-
2
4
3
-
13
-
-
1
-
-
-
1
-
28
3
-
-
-
-
1
-
-
1
-
-
-
6
-
-
1
-
-
-
-
2
-
1
-
2
4
3
-
-
-
1
-
-
1
-
28
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
437892
Blakley
Ribonucleoside triphosphate re ...
Astasia sp., Clostridium sp., Corynebacterium sp., Euglena sp., Lactobacillus leichmannii, Pseudomonas sp., Rhizobium sp.
Methods Enzymol.
51
246-259
1978
4
-
-
-
-
1
6
5
-
6
1
7
-
8
-
-
1
-
-
-
1
1
25
1
-
-
-
-
-
-
-
9
-
-
-
4
-
-
9
-
-
1
-
6
-
5
-
6
1
7
-
-
-
1
-
-
1
1
25
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437890
Chen
Substrate and effector binding ...
Lactobacillus leichmannii
Biochemistry
13
654-661
1974
4
-
-
-
-
-
-
-
-
-
7
2
-
2
-
-
1
-
-
-
1
1
3
1
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
7
2
-
-
-
1
-
-
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437891
Hamilton
Ribonucleotide reductase from ...
Escherichia coli, Euglena gracilis, Euglena gracilis Z, Lactobacillus leichmannii
J. Biol. Chem.
249
4428-4434
1974
11
-
-
-
-
-
2
4
-
2
4
3
-
24
-
-
3
-
-
-
1
-
23
1
-
-
-
-
-
-
-
1
-
-
-
11
-
-
1
-
-
-
-
2
-
4
-
2
4
3
-
-
-
3
-
-
1
-
23
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437889
Sando
Ribonucleotide reductase from ...
Lactobacillus leichmannii, Thermus aquaticus, Thermus aquaticus YT-1, Thermus sp., Thermus sp. X-1
Biochemistry
12
3316-3322
1973
1
-
-
-
-
-
-
5
-
2
1
4
-
25
-
-
2
-
-
-
-
1
35
1
2
2
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
5
-
2
1
4
-
-
-
2
-
-
-
1
35
1
2
2
-
-
1
-
-
-
-
-
-
-
-
-
681889
Yau
The Bacillus megaterium ribonu ...
Bacillus megaterium, Bacillus megaterium KM / ATCC 13632
Mol. Cell. Biochem.
1
101-105
1973
1
-
-
-
-
-
1
-
-
1
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437888
Goulian
Purification and properties of ...
Lactobacillus leichmannii
J. Biol. Chem.
241
4233-4242
1966
3
-
-
-
-
-
2
3
-
-
1
1
-
2
-
-
1
-
-
-
1
3
11
-
-
-
-
-
-
-
-
3
-
-
-
3
-
-
3
-
-
-
-
2
-
3
-
-
1
1
-
-
-
1
-
-
1
3
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437887
Bakley
Cobamides and ribonucleotide r ...
Lactobacillus leichmannii
J. Biol. Chem.
240
2173-2180
1965
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