Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-deoxyadenosylcobalamin | - |
Euglena gracilis | |
5'-deoxyadenosylcobalamin | - |
Lactobacillus leichmannii | |
ATP | stimulates CTP reduction | Euglena gracilis | |
dATP | specific activator for CTP reduction | Euglena gracilis | |
dATP | specific activator for CTP reduction | Lactobacillus leichmannii | |
dCTP | - |
Euglena gracilis | |
dCTP | - |
Lactobacillus leichmannii | |
dGTP | - |
Euglena gracilis | |
dGTP | - |
Lactobacillus leichmannii | |
dTTP | - |
Euglena gracilis | |
dTTP | - |
Lactobacillus leichmannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | inhibits CTP, ATP and GTP reduction, reduction of UTP is unaffected by deoxyribonucleotides | Euglena gracilis | |
dTTP | - |
Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
CTP | in presence of dATP | Euglena gracilis | |
0.15 | - |
CTP | in presence of dTTP | Euglena gracilis | |
0.25 | - |
ATP | in presence of dTTP | Euglena gracilis | |
0.44 | - |
ATP | - |
Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | can stimulate the reaction | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, SDS-PAGE | Euglena gracilis |
140000 | 150000 | - |
Euglena gracilis |
240000 | 278000 | - |
Escherichia coli |
440000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside triphosphate + reduced thioredoxin + H2O | Escherichia coli | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Euglena gracilis | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Euglena gracilis | - |
- |
- |
Euglena gracilis Z | - |
- |
- |
Lactobacillus leichmannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Euglena gracilis |
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.167 | - |
- |
Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + reduced thioredoxin | - |
Escherichia coli | dADP + oxidized thioredoxin + H2O | - |
? | |
ADP + reduced thioredoxin | - |
Lactobacillus leichmannii | dADP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Euglena gracilis | dATP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Lactobacillus leichmannii | dATP + oxidized thioredoxin + H2O | - |
? | |
ATP + reduced thioredoxin | - |
Euglena gracilis Z | dATP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced thioredoxin | - |
Escherichia coli | dCDP + oxidized thioredoxin + H2O | - |
? | |
CDP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCDP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Euglena gracilis | dCTP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dCTP + oxidized thioredoxin + H2O | - |
? | |
CTP + reduced thioredoxin | - |
Euglena gracilis Z | dCTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Euglena gracilis | dGTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dGTP + oxidized thioredoxin + H2O | - |
? | |
GTP + reduced thioredoxin | - |
Euglena gracilis Z | dGTP + oxidized thioredoxin + H2O | - |
? | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Euglena gracilis | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Euglena gracilis Z | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Escherichia coli | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Euglena gracilis | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
UTP + reduced thioredoxin | - |
Euglena gracilis | dUTP + oxidized thioredoxin + H2O | - |
? | |
UTP + reduced thioredoxin | - |
Lactobacillus leichmannii | dUTP + oxidized thioredoxin + H2O | - |
? | |
UTP + reduced thioredoxin | - |
Euglena gracilis Z | dUTP + oxidized thioredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, SDS-PAGE | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | can utilize NADPH as hydrogen donor for ribonucleotide reduction | Euglena gracilis |