Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP maximally stimulates CDP reduction at 1.5 mM | Bacillus subtilis | |
dATP | dATP maximally stimulates CDP reduction at 8-10 microM followed by rapid inhibition at higher concentrations | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dATP | dATP maximally stimulates CDP reduction at 8-10 microM followed by rapid inhibition at higher concentrations | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | may substitute or manganese | Bacillus subtilis | |
Manganese | dimanganic-tyrosyl radical cofactor | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49600 | - |
isolated subunit NrdF, plus major fraction of 80100 Dalton, gel filtration | Bacillus subtilis |
80100 | - |
isolated subunit NrdF, plus small fraction of 49600 Dalton, gel filtration | Bacillus subtilis |
82700 | - |
isolated subunit NrdE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis JH624 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isolation of beta-subunit NrdF using anion exchange chromatography to separate apo-/mismetalated-NrdFs from dimanganic-tyrosyl radical cofactor | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52 | - |
iron-loaded enzyme, cosubstrate dithiothreitol, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
106 | - |
iron-loaded enzyme, cosubstrate thioredoxin YosR, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
125 | - |
iron-loaded enzyme, cosubstrate thioredoxin A, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
146 | - |
manganese-loaded enzyme, cosubstrate dithiothreitol, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
997 | - |
manganese-loaded enzyme, cosubstrate thioredoxin YosR, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
1475 | - |
manganese-loaded enzyme, cosubstrate thioredoxin A, presence of 3 mM ATP, pH 7.6, 37°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + dithiothreitol | - |
Bacillus subtilis | 2'-dCDP + oxidized dithiothreitol + H2O | - |
? | |
CDP + dithiothreitol | - |
Bacillus subtilis JH624 | 2'-dCDP + oxidized dithiothreitol + H2O | - |
? | |
CDP + thioredoxin A | - |
Bacillus subtilis | 2'-dCDP + thioredoxin A disulfide + H2O | - |
? | |
CDP + thioredoxin A | - |
Bacillus subtilis JH624 | 2'-dCDP + thioredoxin A disulfide + H2O | - |
? | |
CDP + thioredoxin YosR | - |
Bacillus subtilis | 2'-dCDP + thioredoxin YosR disulfide + H2O | - |
? | |
CDP + thioredoxin YosR | - |
Bacillus subtilis JH624 | 2'-dCDP + thioredoxin YosR disulfide + H2O | - |
? | |
additional information | Bacillus subtilis ribonucleotide reductase can be assayed as a holo-enzyme by using equivalent amounts of each subunit | Bacillus subtilis | ? | - |
? | |
additional information | Bacillus subtilis ribonucleotide reductase can be assayed as a holo-enzyme by using equivalent amounts of each subunit | Bacillus subtilis JH624 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | at physiological concentrations, alpha-subunit NrdE is a monomer and beta-subunit NrdF in complex with dimanganic-tyrosyl radical cofactor is a dimer. A 1:1 mixture of NrdE:NrdF, however, is composed of a complex mixture of structures | Bacillus subtilis |